Tetramer formation kinetics in the signaling state of AppA monitored by time-resolved diffusion.

Biophysical Journal
Partha HazraMasahide Terazima

Abstract

The photoreaction kinetics of the BLUF domain of AppA(5-125) was studied by monitoring time-dependence of an apparent diffusion coefficient (D) using the pulsed laser-induced transient grating technique. It was found that D of the photoproduct is time-dependent. From the concentration dependence of the reaction rate, it was concluded that the BLUF domain of AppA forms a dimer upon the photoexcitation. Since AppA exists as a dimeric form in the ground state, this dimerization reaction indicates the tetramer formation in the signaling state. From the slope of the plot of observed rate constants (k(obs)) against the AppA concentration, the second order rate constant is determined to be approximately 2.5 x 10(5) M(-1) s(-1), which is approximately 4 orders in magnitude lower than the diffusion controlled reaction. It indicates that a relative orientation of the protein molecules during the dimerization process causes additional constraints, which slow down the reaction rate.

References

Jul 26, 2002·Molecular Microbiology·Stephan BraatschGabriele Klug
Sep 24, 2004·Journal of the American Chemical Society·Yasutaka NishiharaMasahide Terazima
Sep 30, 2004·Biophysical Journal·Keiichi InoueMasahide Terazima
Sep 30, 2004·Biophysical Journal·Shinpei NishidaMasahide Terazima
Sep 22, 2005·Journal of the American Chemical Society·Takeshi EitokuMasahide Terazima
Jan 4, 2006·Biochemistry·Wouter LaanKlaas J Hellingwerf
Jul 21, 2006·The Journal of Physical Chemistry. B·Keiichi InoueMasahide Terazima

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Citations

Nov 22, 2007·Photochemistry and Photobiology·Aba Losi
Aug 31, 2011·Journal of Molecular Biology·Masato KondohMasahide Terazima
May 20, 2009·Journal of Molecular Biology·Keisuke TanakaMasahide Terazima
Nov 28, 2008·Journal of Photochemistry and Photobiology. B, Biology·Jan Götze, Peter Saalfrank
Nov 19, 2015·Frontiers in Molecular Biosciences·Tilo Mathes, Jan P Götze
May 6, 2009·Biophysical Journal·Keiichi InoueMasahide Terazima
Feb 28, 2007·Biophysical Journal·Keiichi InoueMasahide Terazima
Dec 14, 2017·Biophysics Reviews·Tomotsumi Fujisawa, Shinji Masuda
Sep 16, 2008·Chembiochem : a European Journal of Chemical Biology·Claudia SchroederLars-Oliver Essen
Sep 6, 2011·Physical Chemistry Chemical Physics : PCCP·Masahide Terazima
Mar 29, 2013·Photochemical & Photobiological Sciences : Official Journal of the European Photochemistry Association and the European Society for Photobiology·Kunisato KuroiMasahide Terazima
Jan 15, 2008·The Journal of Physical Chemistry. B·Partha HazraMasahide Terazima
May 11, 2007·Journal of the American Chemical Society·Yusuke NakasoneMasahide Terazima
Aug 29, 2006·Biophysical Journal·Takeshi EitokuMasahide Terazima
Apr 1, 2011·The Journal of Physical Chemistry. B·Keiichi InoueHideki Kandori
Aug 13, 2013·The Journal of Physical Chemistry. B·Kimitoshi TakedaMasahide Terazima

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