Tetramethylpyrazine attenuates lipopolysaccharide-induced cardiomyocyte injury via improving mitochondrial function mediated by 14-3-3γ

European Journal of Pharmacology
Bowei HuangMing He

Abstract

Lipopolysaccharide (LPS) is one of the many reasons that can cause myocardial injury. Our previous works have demonstrated that 14-3-3γ could protect myocardium against LPS-induced injury. Tetramethylpyrazine (TMP), an alkaloid found in Chinese herbs, exerts myocardial protection in many ways with multiple targets. We hypothesized that the cardioprotection of TMP against LPS-induced injury is attributed to upregulation of 14-3-3γ and improvement of mitochondrial function. To test the hypothesis, we investigated the effects of TMP on LPS-induced injury to cardiomyocytes by determining cell viability, LDH and caspase-3 activities, reactive oxygen species and MMP levels, mPTP openness, and apoptosis rate. The expression of 14-3-3γ and Bcl-2, and the phosphorylation of Bad (S112) were examined by Western blot. LPS-induced injury to cardiomyocytes was attenuated by TMP via upregulating expression of 14-3-3γ, and Bcl-2 on mitochondria, activating Bad (S112) phosphorylation, increasing cell viability and MMP levels, decreasing LDH and caspase-3 activity, reactive oxygen species generation, mPTP opening and apoptosis rate. However, the cardioprotection of TMP was attenuated by pAD/14-3-3γ-shRNA, an adenovirus that knocked down intracel...Continue Reading

Citations

Jun 20, 2019·Artificial Cells, Nanomedicine, and Biotechnology·Dong ChenPing Sui
Jun 22, 2019·Artificial Cells, Nanomedicine, and Biotechnology·Qi RenChangjie Ren
Jan 8, 2020·International Journal of Molecular Sciences·Abdalla AbdrabouZhixiang Wang
Jun 8, 2021·Acta Pharmaceutica Sinica. B·Yanan HeDingkun Zhang
Oct 28, 2019·Experimental and Molecular Pathology·Qiang LiHongbin Sun

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