Tetramethylpyrazine prevents ethanol-induced hepatocyte injury via activation of nuclear factor erythroid 2-related factor 2

Life Sciences
Chunfeng LuShizhong Zheng

Abstract

Inhibiting the major features of alcoholic liver disease (ALD) such as lipid accumulation and oxidative stress is a promising strategy of treating ALD. Tetramethylpyrazine (TMP) has curative effects on various diseases. However, the effects of TMP on ethanol-induced hepatocyte injury and the related mechanisms remain unclear. The aim of this study is to elucidate the effects of TMP and the potential mechanisms in vitro. Ethanol-stimulated LO2 cells were used as an in vitro model of ALD. Several biomarkers related to cell injury, lipid accumulation, and oxidative stress were determined to evaluate the effects of TMP. Nuclear factor erythroid 2-related factor 2 (Nrf2) expression plasmids and Nrf2 small interfering RNA (siRNA) were used to establish the role of Nrf2. TMP increased Nrf2 expression and nuclear translocation. TMP prevented ethanol-induced hepatocyte injury, as indicated by the enhanced cell viability, reduced activities of aspartate transaminase and alanine aminotransferase in the culture medium, and inhibition of cell apoptosis. Furthermore, TMP reduced the levels of lipid droplets, triglyceride, and total cholesterol, probably by regulating genes related to lipid metabolism. Besides, TMP alleviated ethanol-induced ...Continue Reading

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Citations

Oct 28, 2016·European Journal of Pharmaceutical Sciences : Official Journal of the European Federation for Pharmaceutical Sciences·Huanhuan JinShizhong Zheng
Nov 12, 2016·Toxicological Sciences : an Official Journal of the Society of Toxicology·Chunfeng LuShizhong Zheng
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Jan 11, 2018·Cell Proliferation·Huanhuan JinShizhong Zheng
Aug 10, 2020·Journal of Food Biochemistry·Xiaoxia WangYingbiao Sun
Jun 28, 2018·Cellular and Molecular Life Sciences : CMLS·Ning ZhaoTao Zeng
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Aug 22, 2017·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Chunfeng LuShizhong Zheng
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Sep 25, 2018·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Jing SunJingbo Pi

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