Tetrandrine suppresses lipopolysaccharide-induced microglial activation by inhibiting NF-kappaB pathway

Acta Pharmacologica Sinica
Yang XueLing-yun Xu

Abstract

Microglial activation has been implicated in many neurological diseases. In this study, we examined the effects of tetrandrine (TET), a major pharmacologically-active compound of Chinese herb Stephania tetrandra S Moore on microglial activation. The microglia pretreated with or without TET were activated by lipopolysaccharide (LPS) in vitro. Nitric oxide (NO) release, superoxide anion (O2-) generation, as well as TNF-alpha and interleukin-6 (IL-6) production by microglia were measured afterwards. Electrophoretic mobility shift assay was performed to determine whether NF-kappaB activity in microglia was affected by TET treatment. We found that TET inhibited the LPS-induced activation of microglia by decreasing the production of NO and O2-, consequently affecting the release of TNF-alphaand IL-6 in LPS-induced microglial activation. Such suppressive effect was accompanied by inhibiting transcription factor NF-kappaB activation. Our results suggest that TET might modulate LPS-induced microglial activation by inhibiting the NF-kappaB-mediated release of inflammatory factors.

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Citations

Sep 19, 2014·The International Journal of Neuroscience·Yinghui ChenGuoxiong Xu
Feb 24, 2016·Phytochemistry·N Bhagya, K R Chandrashekar
Aug 31, 2010·Journal of Ethnopharmacology·Deepak Kumar SemwalUsha Rawat
Mar 26, 2016·Frontiers in Pharmacology·M Akhtar AnwarAli H Eid
Jan 23, 2013·Pharmaceutical Biology·Suganthy NatarajanPandima Devi Kasi
Feb 26, 2011·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Dong Kug ChoiKyoungho Suk
Apr 28, 2020·Phytochemistry Reviews : Proceedings of the Phytochemical Society of Europe·Yueping JiangShao Liu
Nov 17, 2020·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Tapan BehlSimona Bungau
Apr 14, 2021·Phytotherapy Research : PTR·Bhawna Chopra, Ashwani Kumar Dhingra
Jun 4, 2021·International Journal of Nanomedicine·Liandi HuangZhiyu Du
Jul 12, 2021·Phytomedicine : International Journal of Phytotherapy and Phytopharmacology·Defang RenZhi Li

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