Tetrazolium-based plaque assay for HIV-1 and HIV-2, and its use in the evaluation of antiviral compounds.

Journal of Virological Methods
H NakashimaE De Clercq

Abstract

A modification of the MT-4 cell plaque assay for human immunodeficiency virus (HIV) is described, which gave reproducible results with all 4 HIV-1 strains and the two HIV-2 strains that were used. The main feature of this new method is the use of a tetrazolium (MTT) staining procedure. The number of plaques read after 4-6 days was essentially the same as the number of infectious units derived from the 50% cell culture infective dose (CCID50) in MT-4 suspension cultures. For a selected group of antiviral compounds the 50% plaque-inhibitory doses were comparable with the 50% inhibitory doses (ID50) in suspension cultures. In the plaque assay HIV-1 (HTLV-IIIB) and HIV-2 (LAV-2ROD) were equally susceptible to azidothymidine (AZT), and the same was true for didehydrodideoxythymidine (D4T). For these compounds it was irrelevant whether they were already present during the initial HIV adsorption phase or added immediately thereafter. Pentosan polysulfate proved about 20-fold more inhibitory to HIV-2 than HIV-1. There was a 5-fold increase in activity if present during the virus adsorption stage.

References

Aug 1, 1988·Journal of Virological Methods·R PauwelsE De Clercq
Jun 1, 1987·Journal of Virological Methods·R PauwelsM Vandeputte
May 9, 1986·Science·J CoffinP Vogt
Aug 1, 1987·Journal of Medicinal Chemistry·P HerdewijnH Vanderhaeghe

❮ Previous
Next ❯

Citations

Jun 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·E De ClercqD Schwartz
Feb 24, 2001·Nucleosides, Nucleotides & Nucleic Acids·T HiratouH Takaku
Apr 29, 1997·Proceedings of the National Academy of Sciences of the United States of America·A GervaixJ Corbeil
Apr 1, 1992·Ecotoxicology and Environmental Safety·F GalganiF Gilbert
Feb 25, 2005·Molecular Pharmacology·Dirk DaelemansErik De Clercq
Dec 6, 2002·Drugs·Susan M Cheer, Karen L Goa
Dec 14, 1999·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·A SavarinoU Dianzani
Apr 2, 1999·Alimentary Pharmacology & Therapeutics·J M SubhaniJ S Dooley
Dec 1, 1993·Antimicrobial Agents and Chemotherapy·M J OttoD L Winslow

❮ Previous
Next ❯

Related Concepts

Related Feeds

Antivirals

Antivirals are medications that are used specifically for treating viral infections. Discover the latest research on antivirals here.

Antivirals (ASM)

Antivirals are medications that are used specifically for treating viral infections. Discover the latest research on antivirals here.