PMID: 9164864May 15, 1997Paper

TFIIF, a basal eukaryotic transcription factor, is a substrate for poly(ADP-ribosyl)ation

The Biochemical Journal
J M Rawling, R Alvarez-Gonzalez

Abstract

We have examined the susceptibility of some of the basal eukaryotic transcription factors as covalent targets for poly(ADP-ribosyl)ation. Human recombinant TATA-binding protein, transcription factor (TF)IIB and TFIIF (made up of the 30 and 74 kDa RNA polymerase II-associated proteins RAP30 and RAP74) were incubated with calf thymus poly(ADP-ribose) polymerase and [32P]NAD+ at 37 degrees C. On lithium dodecyl sulphate/PAGE and autoradiography, two bands of radioactivity, coincident with RAP30 and RAP74, were observed. No radioactivity co-migrated with TATA-binding protein or TFIIB. The phenomenon was dependent on the presence of nicked DNA, which is essential for poly(ADP-ribose) polymerase activity. Covalent modification of TFIIF increased with time of incubation, with increasing TFIIF concentration and with increasing NAD+ concentration. High-resolution PAGE confirmed that the radioactive species associated with RAP30 and RAP74 were ADP-ribose polymers. From these observations, we conclude that both TFIIF subunits are highly specific substrates for covalent poly(ADP-ribosyl)ation.

Citations

Sep 24, 1999·Biological Chemistry·P O Hassa, M O Hottiger
Dec 13, 2003·American Journal of Physiology. Heart and Circulatory Physiology·Basilia ZingarelliBruce J Aronow
Nov 29, 2012·Current Protocols in Chemical Biology·Hong Jiang, Hening Lin
Jul 31, 2001·The Journal of Biological Chemistry·H Mendoza-Alvarez, R Alvarez-Gonzalez
Jan 16, 2007·European Journal of Clinical Investigation·S WagnerA Königsrainer
Feb 3, 2004·American Journal of Respiratory Cell and Molecular Biology·Robert ErtseyLouis M Scavo
Nov 22, 2019·Advances in Wound Care·Jaideep BanerjeeBao-Ngoc Nguyen
Sep 5, 2020·Nature Reviews. Drug Discovery·Nicola J Curtin, Csaba Szabo
Jul 10, 2002·Experimental Dermatology·László VirágCsaba Szabó
Jun 17, 2005·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·Maria Rosaria Faraone-Mennella
Jul 25, 2006·Critical Reviews in Biochemistry and Molecular Biology·Mary C Thomas, Cheng-Ming Chiang
Jul 24, 2008·Virus Genes·Juliana Velasco de Castro OliveiraPaolo Marinho de Andrade Zanotto
Oct 31, 2001·The Journal of Biological Chemistry·Viatcheslav A SoldatenkovAnatoly Dritschilo
May 18, 2000·International Journal of Cancer. Journal International Du Cancer·V A Soldatenkov, M Smulson
Nov 25, 2004·Progress in Biophysics and Molecular Biology·Paul A NguewaJosé M Pérez
Jul 31, 1999·The Journal of Biological Chemistry·M RossignolJ M Egly
Jun 22, 2010·Journal of the American Chemical Society·Hong JiangHening Lin
Aug 17, 1999·Biochemical and Biophysical Research Communications·M KameokaK Yoshihara
Jul 16, 1999·Biochemical and Biophysical Research Communications·M KameokaK Yoshihara
Aug 9, 2001·Neoplasia : an International Journal for Oncology Research·C M Simbulan-RosenthalM E Smulson
Feb 14, 2004·Journal of Cell Science·Michèle RouleauGuy G Poirier
Dec 7, 2007·Science's STKE : Signal Transduction Knowledge Environment·Rafael Alvarez-Gonzalez

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.