TFT and 6TG resistance of mouse lymphoma cells to analogs of azacytidine.

Carcinogenesis
D McGregorW J Caspary

Abstract

5-Azacytidine (5-aza-CR) and six of its analogs were examined for their ability to induce trifluorothymidine (TFT) and/or 6-thioguanine (6TG) resistance in L5178Y mouse lymphoma cells. These analogs were 5-aza-2'-deoxycytidine (5-aza-CdR), 5-fluoro-2'-deoxycytidine (5-FCdR), 5,6-dihydro-5-azacytidine (dH-aza-CR), 6-azacytidine (6-aza-CR), cytidine (CR) and 1-b-D-arabinofuranosylcytosine (ara-C). 5-Aza-CR and 6-aza-CR were examined for their ability to induce 6TG-resistant colonies and results demonstrated no effect. At least a 5-fold increase in TFT resistance was observed for 5-aza-CR, 5-aza-CdR, 5-FCdR, dH-aza-CR and ara-C. The concentration at which these compounds induced TFT resistance correlated well with the potential of the nucleoside analogs to induce differentiation in C3H10T1/2 cells as determined by Constantinides et al. (Nature, 267, 364-366, 1977). In L5178Y mouse lymphoma (MOLY) cells, 5-aza-CR induced TFT resistance and produced both small and large colonies. Previous studies using mammalian cells showed the absence of mutagenic activity with 5-aza-CR and some of its analogs at the ATPase and hgprt loci. However, the different spectrum of DNA lesions detected at the tk locus may be responsible for the response o...Continue Reading

Citations

Dec 1, 1994·Mutation Research·K NegishiH Hayatsu
Aug 1, 1995·Toxicology in Vitro : an International Journal Published in Association with BIBRA·S KirchnerH Stopper
Feb 13, 2001·International Journal of Biological Macromolecules·G Boos, H Stopper
Nov 18, 2009·Biochemistry. Biokhimii︠a︡·O V KirsanovaE S Gromova
Nov 10, 2004·Seminars in Hematology·Yogen Saunthararajah, Joseph DeSimone
Oct 8, 2005·Seminars in Oncology·David S Schrump, Dao M Nguyen
Jan 1, 1996·Environmental and Molecular Mutagenesis·D L SpencerK R Tindall
Aug 26, 2004·British Journal of Haematology·Yogen SaunthararajahJoseph DeSimone
Oct 4, 2011·Journal of Biomolecular Screening·Jason M FoulksSteven B Kanner
Mar 19, 1999·Molecular and Cellular Biology·L BrodayM Costa
Oct 2, 2004·Human Molecular Genetics·Vera GorbunovaJohn H Wilson

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