TGF-β activity in acid bone lysate adsorbs to titanium surface

Clinical Implant Dentistry and Related Research
Franz Josef StraussReinhard Gruber

Abstract

Osteoblasts lay down new bone on implant surfaces. The underlying cellular mechanism and the spatio-temporal mode of action, however, remain unclear. It can be proposed that growth factors released upon acidification by osteoclasts adsorb to the implant surface and control the early stages of osseointegration. To simulate bone lysis by osteoclasts, titanium discs were exposed to acid bone lysate (ABL) followed by vigorous washing and seeding of oral fibroblasts. The expression of TGF-β target genes interleukin 11 (IL11) and NADPH oxidase 4 (NOX4) was evaluated by reverse transcriptase polymerase chain reaction and IL11 ELISA. TGF-β signaling activation was assessed via Smad2/3 immunofluorescence. The impact of ABL on osteogenic differentiation was determined with murine ST2 mesenchymal stromal cells. We report here that ABL-conditioned titanium discs, independent of turned or rough surface, increased the expression of IL11 and NOX4. This increase was blocked by the TGF-β receptor 1 antagonist SB431542. Further support for the TGF-β signaling activation came from the translocation of Smad2/3 into the nucleus of oral fibroblasts. Moreover, titanium discs exposed to ABL decreased alkaline phosphatase and osteopontin in ST2 cells. ...Continue Reading

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Oct 31, 2018·Scientific Reports·Franz Josef StraussReinhard Gruber

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Citations

Jul 2, 2020·Journal of Biomedical Materials Research. Part a·Franz-Josef StraussReinhard Gruber
Jun 25, 2020·Scientific Reports·Francesca Di SummaFranz-Josef Strauss
Dec 6, 2020·International Journal of Molecular Sciences·Jila NasirzadeReinhard Gruber
Jun 12, 2021·Scientific Reports·Jila NasirzadeReinhard Gruber

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Methods Mentioned

BETA
chips
PCR
Reverse transcription PCR
ELISA

Software Mentioned

Prism
GraphPad

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