TGF-β causes Docetaxel resistance in Prostate Cancer via the induction of Bcl-2 by acetylated KLF5 and Protein Stabilization.

Theranostics
Yixiang LiJin-Tang Dong

Abstract

Prostate cancer is the second leading cause of cancer-related death in the United States. As a first line treatment for hormone-refractory prostate cancer, docetaxel (DTX) treatment leads to suboptimal effect since almost all patients eventually develop DTX resistance. In this study, we investigated whether and how TGF-β affects DTX resistance of prostate cancer. Methods: Cytotoxicity of DTX in DU 145 and PC-3 cells was measured by CCK-8 and Matrigel colony formation assays. Resistance to DTX in DU 145 cells was examined in a xenograft tumorigenesis model. A luciferase reporter system was used to determine transcriptional activities. Gene expression was analyzed by RT-qPCR and Western blotting. Results: We found that KLF5 is indispensable in TGF-β-induced DTX resistance. Moreover, KLF5 acetylation at lysine 369 mediates DTX resistance in vitro and in vivo. We showed that the TGF-β/acetylated KLF5 signaling axis activates Bcl-2 expression transcriptionally. Furthermore, DTX-induced Bcl-2 degradation depends on a proteasome pathway, and TGF-β inhibits DTX-induced Bcl-2 ubiquitination. Conclusion: Our study demonstrated that the TGF-β-acetylated KLF5-Bcl-2 signaling axis mediates DTX resistance in prostate cancer and blockade of t...Continue Reading

Citations

Jun 28, 2021·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Milad AshrafizadehSaeed Samarghandian
Jul 25, 2021·International Journal of Molecular Sciences·Jeff Yat-Fai ChungPatrick Ming-Kuen Tang

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Methods Mentioned

BETA
acetylation
transfection
PCR
Assay
flow cytometry
immunoprecipitation
Protein Assay
xenograft
pull down
ubiquitination

Clinical Trials Mentioned

NCT01401062

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