Nov 8, 2018

TGF-β Mediated Cell Adhesion Dynamics and Epithelial to Mesenchymal Transition in 3D and 2D Ovarian Cancer Models

BioRxiv : the Preprint Server for Biology
Wafaa Al AmeriThasni Karedath

Abstract

Background: Three-dimensional (3D) tumor cultures in vitro have recently regained attention as they have been acclaimed to have higher similarity to in vivo tumors than cells grown in conventional monolayers (2D). Cancer cells in 2D monolayers fail to mimic the exact tumor microenvironment existing in the real clinical samples. The 3D anchorage-independent cancer models can better explain the cell adhesion properties in ovarian cancer cells. Results: Transcriptome profiling of 3D models compared to 2D models in various cancer cell lines shows differential expression of TGF-β-mediated and cell adhesion pathways. Presence of TGF-β in these cell lines shows an increased invasion potential which is specific to cell type. We decided to choose two ovarian cancer cell lines as model systems to study Epithelial to Mesenchymal Transition (EMT) and cancer invasion based on their invasion potential and ability to switch the cadherins expression. TGF-β modulation in EMT and cancer invasion was successfully depicted in both 2D and 3D models of SKOV3 and CAOV3 cell lines. Functional evaluations in 3D and 2D models demonstrate that the addition of the exogenous TGF-β can induce EMT and invasion in cancer cells by turning them into aggressive ...Continue Reading

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Mentioned in this Paper

Metabolic Process, Cellular
In Vivo
Biochemical Pathway
Animal Cancer Model
Transforming Growth Factor beta
2-Dimensional
TGFA protein, human
Three-dimensional
Cell to Cell Adhesion Signaling Pathway
Extracellular Matrix

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