Abstract
Adult neural progenitor cells (NPCs) are capable of differentiating into neurons, astrocytes, and oligodendrocytes throughout life. Notch and transforming growth factor β1 (TGF-β) signaling pathways play critical roles in controlling these cell fate decisions. TGF-β has been previously shown to exert pro-neurogenic effects on hippocampal and subventricular zone (SVZ) NPCs in vitro and to interact with Notch in different cellular types. Therefore, the aim of our work was to study the effect of TGF-β on adult rat brain SVZ NPC glial commitment and its interaction with Notch signaling. Initial cell characterization revealed a large proportion of Olig2+, Nestin+, and glial fibrillary acidic protein (GFAP+) cells, a low percentage of platelet-derived growth factor receptor α (PDGFRα+) or NG2+ cells, and <1% Tuj1+ cells. Immunocytochemical analyses showed a significant increase in the percentage of PDGFRα+, NG2+, and GFAP+ cells upon four-day TGF-β treatment, which demonstrates the pro-gliogenic effect of this growth factor on adult brain SVZ NPCs. Real-time polymerase chain reaction analyses showed that TGF-β induced the expression of Notch ligand Jagged1 and downstream gene Hes1. Notch signaling inhibition in cultures treated with ...Continue Reading
References
May 22, 1988·The Journal of Comparative Neurology·G F McCarthy, C P Leblond
Aug 15, 1970·Nature·U K Laemmli
May 20, 1994·Science·C Lois, A Alvarez-Buylla
Nov 11, 1998·Nature Medicine·P S ErikssonF H Gage
Dec 14, 1999·The European Journal of Neuroscience·B Nait-OumesmarA Baron-Van Evercooren
Apr 11, 2001·Proceedings of the National Academy of Sciences of the United States of America·K JinD A Greenberg
Sep 18, 2002·Proceedings of the National Academy of Sciences of the United States of America·Nathalie Picard-RieraAnne Baron-Van Evercooren
Oct 3, 2002·Nature Medicine·Gareth R JohnCelia F Brosnan
Apr 15, 2004·Molecular and Cellular Neurosciences·M A HackM Götz
Aug 25, 2004·Glia·Steven PetratosTrevor J Kilpatrick
Jan 20, 2006·The European Journal of Neuroscience·Daniela BattistaFernando J Pitossi
Jul 28, 2006·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Bénédicte MennArturo Alvarez-Buylla
Mar 29, 2007·Brain Pathology·Hans LassmannClaudia F Lucchinetti
Aug 19, 2007·Science's STKE : Signal Transduction Knowledge Environment·Krit KitisinLopa Mishra
Oct 27, 2007·Journal of the Neurological Sciences·B Nait-OumesmarA Baron-Van Evercooren
Aug 19, 2008·Glia·Alexandre I DanilovOlle Lindvall
Dec 31, 2008·Cell Research·Katharine H WrightonXin-Hua Feng
Apr 22, 2009·Cell·Raphael Kopan, Maria Xenia G Ilagan
Feb 6, 2010·Neuroimmunomodulation·Patricia MathieuFernando Pitossi
Jun 26, 2010·Stem Cells and Development·Andrew P RobinsonDarwin J Prockop
Jan 18, 2011·Brain Research Reviews·Oscar Gonzalez-Perez, Arturo Alvarez-Buylla
Mar 8, 2011·PloS One·Richa K DaveBrandon J Wainwright
Jun 5, 2013·Brain, Behavior, and Immunity·Mariana GraciarenaFernando J Pitossi
Sep 17, 2013·Journal of Neurochemistry·Evangelina AparicioAna María Adamo
Jun 6, 2014·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Javier PalazuelosAdan Aguirre
Mar 19, 2015·Glia·Fuzheng GuoDavid Pleasure
Jun 9, 2015·Molecular and Cellular Neurosciences·Pablo Daniel RadiceFernando Juan Pitossi