Abstract
It is generally thought that the proliferative capacity and differentiation potential of somatic stem cells, including mesenchymal stromal/stem cells (MSCs) and hematopoietic stem cells, decline with age. We investigated the effects of aging on human bone-derived MSCs expressing CD271 and SSEA-4 (double-positive MSCs [DPMSCs]). The percentages of DPMSCs in bone tissue decreased significantly with age. The DPMSCs from elderly patients (old DPMSCs) showed cellular senescence, which was evidenced by low growth potential, high senescence-associated β-galactosidase activity, and elevated p16 and p21 CDK inhibitor levels. Moreover, old DPMSCs showed weak osteogenic differentiation potential and less hematopoiesis-supporting activity in comparison with young DPMSCs. Interestingly, the addition of transforming growth factor β2 (TGF-β2) induced cellular senescence in young DPMSCs. With the exception of the adipogenic differentiation potential, all of the aging phenomena observed in old DPMSCs were reversed by the addition of anti-TGF-β antibodies. These results suggest that, in part, old DPMSCs accelerate cellular senescence through TGF-β signaling.
Citations
Nov 22, 2018·Journal of Experimental Zoology. Part B, Molecular and Developmental Evolution·Xin-Xin ZhaoXue-Ming Zhang
Mar 29, 2019·Journal of Cellular and Molecular Medicine·Haiwei HeXin Li
Sep 19, 2019·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Sajjad AshrafRita Kandel
Nov 28, 2018·Biological Procedures Online·Marco MalavoltaMauro Provinciali
Mar 5, 2019·Aging Cell·Daniela GnaniRaffaella Di Micco
Aug 31, 2019·Stem Cells International·Payal GangulyElena A Jones
Oct 30, 2019·International Journal of Molecular Sciences·Kana Tominaga, Hiroshi I Suzuki
Jul 19, 2020·Biomedicines·Payal GangulyElena A Jones
Aug 11, 2020·Oxidative Medicine and Cellular Longevity·Yan XuJian-Hui Xiao
Jan 11, 2019·Cell & Bioscience·Yiming WangMing Pei
Oct 6, 2018·The Journals of Gerontology. Series A, Biological Sciences and Medical Sciences·Edgar Ledesma-MartínezEdelmiro Santiago-Osorio
Feb 8, 2020·Cells·Francisco Triana-MartínezEduardo Domínguez
Feb 16, 2021·Pharmacology & Therapeutics·Dingyu HuJi Zhang
Dec 3, 2020·Cells·Augustin M OfiteruJorge S Burns
Jun 9, 2021·Scientific Reports·Vera GrotheerJan Grassmann