TGF-β1 induced fascin1 expression facilitates the migration and invasion of kidney carcinoma cells through ERK and JNK signaling pathways

Biochemical and Biophysical Research Communications
Jianyu YangChuize Kong

Abstract

Transforming growth factor-β1 (TGF-β1) plays a crucial role in the signaling network that controls cellular invasion and motility capability during tumor development. To investigate whether fascin1 plays a crucial role in TGF-β1-facilitated invasion and migration of kidney cancer cells (KCC), real-time PCR and western blotting were used to test the fascin1 expression after TGF-β1 treatment (10 ng/ml) in 769-P and OSRC cells. Fascin1 was silenced using the small interfering RNA (siRNA) technique. Cytoskeleton staining was used to test the change of Cytoskeleton. Cell migration and invasion changes were measured by wound-healing and Transwell assay. The results indicate that mRNA and protein levels of fascin1 were dramatically increased after treatment with 10 ng/ml TGF-β1 in 769-P and OSRC cells. TGF-β1 promoted the occurrence of EMT (Epithelial-Mesenchymal Transition) and the invasive and migratory capabilities of the two cell lines after treatment with 10 ng/ml TGF-β1. In addition, fascin1 siRNA dramatically attenuated the invasiveness and migration induced by TGF-β1. Furthermore, we identified that specific inhibitors of ERK and JNK signaling pathways, FR180204 and SP600125, can suppress TGF-β1-induced fascin1 expression. In ...Continue Reading

Citations

Dec 20, 2018·American Journal of Physiology. Renal Physiology·Yingfeng ShiNa Liu
Jul 14, 2020·The FEBS Journal·Shengchen LinShengyu Yang
Dec 31, 2019·BioMed Research International·Qing-Ling ZhangChang-Deok Kim
Feb 23, 2021·Molecular Therapy Oncolytics·Hongliang LiuWei Gao
Apr 6, 2021·Frontiers in Cell and Developmental Biology·Qi YanJian Du
May 24, 2021·Cancer Treatment and Research Communications·Ignacy Górecki, Beata Rak
Jun 9, 2021·Comparative Biochemistry and Physiology. Toxicology & Pharmacology : CBP·Na LiMeiling Hong

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