TGF-β1 mediated MAPK signaling pathway promotes collagen formation induced by Nano NiO in A549 cells

Environmental Toxicology
Minmin TianYingbiao Sun

Abstract

Nickel oxide nanoparticles (Nano NiO) could induce pulmonary fibrosis, however, the mechanisms are still unknown. The aim of the present study was to explore the roles of transforming growth factor-β1 (TGF-β1), mitogen-activated protein kinase (MAPK) pathway and MMPs/TIMPs balance in Nano NiO-induced pulmonary fibrosis. For that purpose, we first established Nano NiO-induced human lung adenocarcinoma epithelial cells (A549 cells) model of collagen excessive formation through detecting the levels of hydroxyproline (Hyp) and type I collagen (Col-I). Then the protein levels of TGF-β1, MAPKs, and MMPs/TIMPs were assessed by Western blot. The results showed that Nano NiO resulted in the increased contents of Hyp, Col-I, and TGF-β1, the MAPK pathway activation and MMPs/TIMPs imbalance with a dose-dependent manner. In addition, to investigate whether TGF-β1 mediated MAPK signaling pathway, A549 cells were treated by 100 μg/mL Nano NiO combined with TGF-β1, p38 MAPK, and ERK1/2 inhibitors (10 μM SB431542, 10 μM SB203580, and 10 μM U0126), respectively. We found that MAPK signal pathway was suppressed by TGF-β1 inhibitor. Meanwhile, the increased contents of Hyp and Col-I, and MMPs/TIMPs imbalance were alleviated by the p38 MAPK and ERK...Continue Reading

References

Nov 9, 2000·American Journal of Respiratory and Critical Care Medicine·M SugaM Ando
Jun 11, 2005·Respiratory Research·Hidenori KasaiZhi Zhang
Sep 21, 2005·Brazilian Journal of Medical and Biological Research = Revista Brasileira De Pesquisas Médicas E Biológicas·V LagenteE Boichot
Aug 31, 2007·Journal of Cellular Biochemistry·Rod A Rahimi, Edward B Leof
Jan 15, 2011·Journal of Occupational Health·Masanori HorieHitoshi Iwahashi
Jun 28, 2011·The European Respiratory Journal·R C A DancerD R Thickett
Dec 6, 2011·Breast Cancer : the Journal of the Japanese Breast Cancer Society·Takeshi ImamuraYasumichi Inoue
Feb 3, 2012·International Journal of Biological Sciences·Guiqian ChenYi-Ping Li
Jun 2, 2012·Frontiers in Bioscience (Landmark Edition)·Li XiaoZhenhua Li
Aug 2, 2012·The Journal of Clinical Investigation·Paul W NobleDianhua Jiang
Oct 27, 2012·The European Respiratory Journal·Bruce D UhalMaria Molina-Molina
Feb 7, 2013·Human & Experimental Toxicology·I-C LeeJ-C Kim
Jan 1, 2014·Laboratory Investigation; a Journal of Technical Methods and Pathology·Ju-Hyun GongYoung-Hee Kang
Feb 8, 2014·Toxicology Letters·Laura CapassoMaurizio Gualtieri
Feb 24, 2015·Journal of Cellular and Molecular Medicine·Xue ZhouChang-Jun Lv
Mar 25, 2016·Journal of Molecular Histology·Ruth MagayeJinshun Zhao
Jul 20, 2016·Tuberculosis and Respiratory Diseases·Ae Rin BaekChoon Sik Park
Sep 7, 2016·Human & Experimental Toxicology·X H ChangY B Sun
Sep 28, 2016·Medical Science Monitor : International Medical Journal of Experimental and Clinical Research·Xuejun AnYonghui Wu
Mar 31, 2017·Medical Science Monitor : International Medical Journal of Experimental and Clinical Research·Zuowei LiFrançoise Ndinga Andely
Mar 9, 2018·Matrix Biology : Journal of the International Society for Matrix Biology·Awen MenouBruno Crestani
Jun 6, 2018·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Shengqi ZhangDeshan Li
Jul 5, 2018·Journal of Cellular and Molecular Medicine·Weibin QianDongli Wang
Feb 8, 2017·Toxicology Research·Fangfang LiuYingbiao Sun

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Citations

Apr 26, 2021·Toxicological Sciences : an Official Journal of the Society of Toxicology·Haibing ZhanYingbiao Sun

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