TGF-β1-Modified Hyaluronic Acid/Poly(glycidol) Hydrogels for Chondrogenic Differentiation of Human Mesenchymal Stromal Cells

Macromolecular Bioscience
Thomas BöckJürgen Groll

Abstract

In cartilage regeneration, the biomimetic functionalization of hydrogels with growth factors is a promising approach to improve the in vivo performance and furthermore the clinical potential of these materials. In order to achieve this without compromising network properties, multifunctional linear poly(glycidol) acrylate (PG-Acr) is synthesized and utilized as crosslinker for hydrogel formation with thiol-functionalized hyaluronic acid via Michael-type addition. As proof-of-principle for a bioactivation, transforming growth factor-beta 1 (TGF-β1) is covalently bound to PG-Acr via Traut's reagent which does not compromise the hydrogel gelation and swelling behavior. Human mesenchymal stromal cells (MSCs) embedded within these bioactive hydrogels show a distinct dose-dependent chondrogenesis. Covalent incorporation of TGF-β1 significantly enhances the chondrogenic differentiation of MSCs compared to hydrogels with supplemented noncovalently bound TGF-β1. The observed chondrogenic response is similar to standard cell culture with TGF-β1 addition with each medium change. In general, multifunctional PG-Acr offers the opportunity to introduce a range of biomimetic modifications (peptides, growth factors) into hydrogels and, thus, ap...Continue Reading

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Citations

Mar 18, 2020·Journal of Tissue Engineering and Regenerative Medicine·Sebastian FrischholzTorsten Blunk
Apr 14, 2020·Materials Science & Engineering. C, Materials for Biological Applications·Tingting WuZhengang Zha
Feb 14, 2021·Materials Science & Engineering. C, Materials for Biological Applications·Matthew WalkerMarco Cantini
Apr 3, 2019·ACS Applied Materials & Interfaces·Yuanfeng ChenZhengang Zha

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