TGF-β1 modulates podocyte migration by regulating the expression of integrin-β1 and -β3 through different signaling pathways

Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie
Chien-An ChenYu-Lin Yang

Abstract

Podocyte migration may lead foot process effacement and proteinuria. Transforming growth factor-β1 (TGF-β1) and integrins are involved in the adhesion and migration of cells. However, the crosstalk of TGF-β1 and integrins is unclear. Here, we examined how TGF-β1 regulates the expression of integrin-β1 and -β3 to modulate podocyte adhesion and migration. Podocytes were exposed to TGF-β1 and/or the inhibitors of Smad2/3, ERK and p38, then the expression of integrin-β1 and -β3 was assessed by Real-time PCR and western blot analyses. Podocyte adhesion and migration were measured under TGF-β1 treatment and/or anti-integrin-β3 antibody by cell adhesion assay and wound healing assay. TGF-β1 had no effect on integrin-β1 mRNA expression. In the analysis of protein expression, TGF-β1 decreased the mature form of integrin-β1, but increased both the precursor form and core peptide of integrin-β1. The inhibitors of ERK and p38, but not Smad2/3, abrogated TGF-β1-induced changes in integrin-β1 protein expression. TGF-β1 increased integrin-β3 mRNA and protein levels. The inhibitors of Smad2/3, ERK and p38 attenuated the TGF-β1-induced increase in integrin-β3 mRNA and protein levels. Podocyte adhesion and migration were enhanced under the stimu...Continue Reading

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Citations

Dec 15, 2020·American Journal of Physiology. Renal Physiology·Wenjun GaoFan Fan
Dec 16, 2018·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Yi-Qi YangPeng Xu
Jan 28, 2020·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Chien-An ChenHung-Chun Chen

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