TGF-β1 Promotes Hepatocellular Carcinoma Invasion and Metastasis via ERK Pathway-Mediated FGFR4 Expression

Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology
Jiawei HuangLin Cai

Abstract

TGF-β1 is beneficial during early liver disease but is tumor-progressive during late stages especially for hepatocellular carcinoma (HCC). Thus, exploring the underlying mechanisms may provide information about a potentially therapeutic role of TGF-β1 in HCC. Western blot and real-time quantitative PCR were used to quantify FGFR4 expression in HCC cell lines and a normal liver cell line. After constructing the best silencing FGFR4 expression vector, migration and invasiveness of TGF-β1 in HCC was studied in vitro and in vivo. Western blot was used to study the mechanism of TGF-β1 induction on FGFR4 expression with various inhibitors. HepG2 cell lines had the most FGFR4 expression, and data show that silencing FGFR4 suppressed cell proliferation, invasion and migration in HCC induced by TGF-β1 in vitro and in vivo. Moreover, TGF-β1 induced FGFR4 expression through the ERK pathway. Promoting FGFR4 expression via the ERK pathway, TGF-β1 contributes to HCC invasion and metastasis.

Citations

Aug 23, 2019·Liver International : Official Journal of the International Association for the Study of the Liver·Peter R GalleAndrew X Zhu
Sep 5, 2019·Journal of Gastroenterology and Hepatology·Sung Whan ChaSoon Koo Baik
Feb 16, 2021·Frontiers in Oncology·Clelia DonisiMario Scartozzi
Jan 20, 2020·Journal of Hepatology·Sandrine FaivreRichard S Finn
Apr 15, 2021·Cancer Cell International·Zahra FarzanehMaryam Farzaneh
May 14, 2021·Frontiers in Pharmacology·Yanan LiuHuanhai Xu

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