TGF-b2, catalase activity, H2O2 output and metastatic potential of diverse types of tumour

BioRxiv : the Preprint Server for Biology
Malak HaidarG Langsley

Abstract

Theileria annulata is a protozoan parasite that infects and transforms bovine macrophages causing a myeloid-leukaemia-like disease called tropical theileriosis. TGF-b2 is highly expressed in many cancer cells and is significantly increased in Theileria-transformed macrophages, as are levels of Reactive Oxygen Species (ROS), notably H2O2. Here, we describe the interplay between TGF-b2 and ROS in cellular transformation. We show that TGF-b2 drives expression of catalase to reduce the amount of H2O2 produced by T. annulata-transformed bovine macrophages, as well as by human lung (A549) and colon cancer (HT-29) cell lines. Theileria-transformed macrophages attenuated for dissemination express less catalase and produce more H2O2, but regain both virulent migratory and matrigel traversal phenotypes when stimulated with TGF-b2, or catalase that reduce H2O2 output. Increased H2O2 output therefore, underpins the aggressive dissemination phenotype of diverse tumour cell types, but in contrast, too much H2O2 can dampen dissemination.

Related Concepts

Catalase
Hydrogen Peroxide
Myeloid Leukemia, Chronic
Lung
Macrophage
Neoplasms
Virulence
Transforming Growth Factor Beta 2
B-Cell Lymphomas
Theileria annulata

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