TGF-beta 1 downregulates CFTR expression and function in nasal polyps of non-CF patients

American Journal of Physiology. Lung Cellular and Molecular Physiology
V Prulière-EscabasseA Coste

Abstract

Nasal polyposis is a chronic inflammatory disease of the upper airways. It has been suggested that ion transports and CFTR expression could be modified in epithelial cells from nasal polyps of non-cystic fibrosis patients. We compared human nasal epithelial cells from nasal polyps (NP) with control nasal mucosa (CM). The level of CFTR mRNA was studied by Northern blot analysis and protein expression was studied by immunoprecipitation both ex vivo and in vitro in primary cultures of human nasal epithelial cells at the air-liquid interface. Ion transports were evaluated by short-circuit measurements in vitro. CFTR gene and protein expressions were significantly decreased in NP native tissues and in culture on day 4, when a global defect of ion transports was observed in NP cultures, but not in CM. We evaluated the effect of transforming growth factor (TGF)-beta 1 on CFTR expression and function in NP cultures on day 14 and showed, for the first time, that TGF-beta 1 was able to significantly downregulate the level of CFTR mRNA and cAMP-dependent current in NP cultures. Finally, we showed that the effects of TGF-beta 1 on ion transports could be reversed after 48-h removal of TGF-beta1 in NP cultures. In conclusion, our data stron...Continue Reading

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Citations

Nov 30, 2010·American Journal of Physiology. Lung Cellular and Molecular Physiology·R Mac SweeneyD F McAuley
Mar 31, 2006·Journal of Inflammation·Michael Eisenhut
Aug 21, 2009·Expert Opinion on Biological Therapy·Jae Woo LeeMichael A Matthay
Mar 12, 2015·International Journal of Oncology·Jin LiHsiao Chang Chan
Jul 1, 2014·American Journal of Respiratory Cell and Molecular Biology·Hoshang J UnwallaMatthias Salathe
Feb 8, 2018·Cellular and Molecular Life Sciences : CMLS·Jieting ZhangHsiao Chang Chan
Jun 23, 2006·Proteomics·Mario OlleroAleksander Edelman
Feb 1, 2011·Pflügers Archiv : European journal of physiology·Michael Eisenhut, Helen Wallace
Sep 28, 2012·Science China. Life Sciences·XiaoHua JiangHsiao Chang Chan
Apr 5, 2015·American Journal of Physiology. Lung Cellular and Molecular Physiology·Parker S WoodsIan C Davis
Jan 23, 2018·Frontiers in Immunology·Jürg HamacherUz Stammberger
Jul 14, 2017·Scientific Reports·Jie Ting ZhangHsiao Chang Chan
Jan 9, 2021·Journal of Cystic Fibrosis : Official Journal of the European Cystic Fibrosis Society·Nikhil T AwatadeShafagh A Waters
May 1, 2021·International Journal of Molecular Sciences·Duncan E Keegan, John J Brewington

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