PMID: 8960384Aug 1, 1996Paper

TGF-beta 1 regulation of laminin secretion by F9-derived primitive endoderm cells: a potential role in cell migration within the mouse blastocyst

Canadian Journal of Physiology and Pharmacology
A M Tonary, J A Carnegie

Abstract

The first differentiation event occurring in the preimplantation mouse blastocyst is the formation of inner cell mass (ICM) derived primitive endoderm (PrE) cells, some of which migrate over the inner surface of the trophectoderm to establish the parietal endoderm layer. Reichert's membrane, a basement membrane located between the trophectoderm and the emerging endodermal layer, is implicated in this migration. In this study, F9 murine embryonal carcinoma cells were used as an in vitro model for embryonic endoderm to investigate the regulation of laminin secretion by these cells during their differentiation under serum-free culture conditions. The formation of PrE-like cells was induced using retinoic acid, and the cells were then cultured with dibutyryl cAMP (dbcAMP), forskolin, or transforming growth factor (TGF) beta 1 or beta 2, and the levels of secreted laminin were measured using ELISA. dbcAMP and forskolin stimulated (p < 0.01) laminin secretion, whereas TGF-beta 1 decreased (p < 0.01) the secretion of laminin by PrE-like cells during a 72-h culture period (without influencing laminin deposition by these cells). In contrast, TGF-beta 2 did not significantly influence (p > 0.05) laminin secretion. The results of in vitro...Continue Reading

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Citations

Jun 1, 2001·Fertility and Sterility·H W ChenC R Tzeng
Apr 8, 2017·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Yue MengXiang-Xi Xu
May 17, 2000·Molecular Reproduction and Development·B A Roelen, C L Mummery

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