PMID: 8950510Nov 1, 1996Paper

TGF-beta 2 increases alpha-smooth muscle actin expression in bovine retinal pigment epithelial cells

Current Eye Research
D KurosakaH Katsura

Abstract

To examine whether transforming growth factor-beta 2 (TGF-beta 2) induces the expression of alpha-smooth muscle actin (SMA), a biochemical marker of myofibroblasts, in cultured bovine retinal pigment epithelial (RPE) cells. Bovine RPE cells were cultured in F-12 nutrient mixture supplemented with 5% fetal bovine serum (FBS), with or without TGF-beta 2 (0.01-10 ng/ml) for 6 days. During this culture period, cells did not reach a confluence. Alpha-SMA was detected immunocytochemically with a mouse monoclonal antibody, and the ratio of the number of alpha-SMA positive cells to the total number of cells was calculated. About 10% of the cells in control cultures with only FBS were positive for alpha-SMA. TGF-beta 2 increased the ratio of positive cells dose-dependently (p < 0.0001), while a neutralizing antibody against TGF-beta 2 blocked this effect. TGF-beta 2 induced expression of alpha-SMA in bovine RPE cells.

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Citations

Apr 13, 2011·Histopathology·Michaela KritzenbergerJost Hillenkamp
Oct 21, 1998·Experimental Eye Research·G S McGillem, R F Dacheux
Jul 4, 2016·Histochemistry and Cell Biology·Andreas OhlmannErnst R Tamm
Jul 27, 2004·Laboratory Investigation; a Journal of Technical Methods and Pathology·Shizuya SaikaAnita B Roberts
Feb 7, 2007·Ophthalmic Surgery, Lasers & Imaging : the Official Journal of the International Society for Imaging in the Eye·Xiao-Bo YuGuy J Ben Simon
Aug 8, 1998·Progress in Retinal and Eye Research·T Kivelä, M Uusitalo

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