PMID: 7541294Jul 1, 1995Paper

TGF-beta and endothelial cells inhibit VCAM-1 expression on human vascular smooth muscle cells

Arteriosclerosis, Thrombosis, and Vascular Biology
J R GambleM A Vadas

Abstract

Vascular smooth muscle cells (VSMCs) are normally devoid of the adhesion protein vascular cell adhesion molecule-1 (VCAM-1), which has, however, been observed on human VSMCs in atheroma. We now show that cultured human saphenous vein VSMCs express small amounts of VCAM-1 and that the cytokine tumor necrosis factor-alpha (TNF-alpha) induces, in a time- and dose-dependent fashion, a significant increase in its expression. Interleukin (IL)-4, IL-1, and to a lesser extent interferon gamma have similar effects. TNF-alpha-stimulated human VSMCs demonstrate increased binding of T lymphocytes that is totally VCAM-1 mediated. The cytokine transforming growth factor-beta (TGF-beta) at 2.0 ng/mL inhibited basal VCAM-1 expression by 84 +/- 8% and the induction by TNF-alpha by between 56 +/- 16% and 77 +/- 15% depending on the dose of TNF. Furthermore, coculture on opposing sides of a polycarbonate filter of human VSMCs with human umbilical vein endothelial cells also inhibited the induction of VCAM-1 by 47 +/- 6%. As active TGF-beta is produced upon the coculture of VSMCs and endothelial cells, we suggest that the close physical proximity of these cells in vivo is responsible for the lack of expression of VCAM-1 on VSMCs and that the inter...Continue Reading

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Citations

Jun 7, 2005·Journal of Huazhong University of Science and Technology. Medical Sciences = Hua Zhong Ke Ji Da Xue Xue Bao. Yi Xue Ying De Wen Ban = Huazhong Keji Daxue Xuebao. Yixue Yingdewen Ban·Wenpei XiangHanping Chen
Mar 23, 2004·Biochemical and Biophysical Research Communications·Kenji IwaiMitsuhiro Yokoyama
Mar 25, 2000·The Journal of Steroid Biochemistry and Molecular Biology·N KikuchiH Honjo
Sep 28, 2002·Current Opinion in Lipidology·Ziad Mallat, Alain Tedgui
Mar 4, 2014·International Journal of Molecular Sciences·Pei-Chen WangKuang-Hung Cheng
May 1, 1997·Clinical and Experimental Pharmacology & Physiology·M A VadasP Barter
Dec 21, 2019·Experimental Cell Research·Marius Andreas JägerThomas Korff
Aug 26, 1998·Biochemical and Biophysical Research Communications·D Bishop-BaileyJ A Mitchell
Feb 3, 2007·Neurobiology of Disease·Henrietta M NielsenSabina M Janciauskiene
Jun 23, 2005·Journal of Agricultural and Food Chemistry·Hyang-Mi KwonYoung-Hee Kang

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