PMID: 2498805May 1, 1989Paper

TGF beta inhibits Go/S-phase transition in primary fibroblasts. Loss of response to the antigrowth effect of TGF beta is observed after immortalization

Oncogene
V Sorrentino, S Bandyopadhyay

Abstract

Transforming growth factor beta (TGF beta) may act as a negative growth regulator in secondary cultures of rodent fibroblasts by preventing quiescent cells from re-entering the cell cycle. Although TGF beta inhibits the serum induced transition from the Go to the S phase it does not inhibit induction of fos, myc, and JE. Actively growing cells are also inhibited in their proliferation by picomolar amounts of TGF beta. In contrast several established cell lines are not inhibited by TGF beta and in these it can cause quiescent cells to resume proliferation. This resistance to TGF beta can be traced back to the early divisions of immortal cells emerging from a senescence crisis, suggesting that alterations in the response of normal fibroblasts to TGF beta are concurrent with an unlimited proliferative potential. Induction of DNA synthesis by TGF beta is preceded in 5 established cell lines by induction of PDGF B chain mRNA as well as of other mRNAs associated with cell replication such as fos, myc, and JE. None of these genes is induced by TGF beta alone in presenescent cells. These results indicate that TGF beta can negatively regulate growth of normal presenescent fibroblasts. Alterations in the responsiveness to TGF beta can be...Continue Reading

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.