PMID: 9186839May 1, 1997Paper

TGF-beta-mediated hepatocellular apoptosis by rat and human hepatoma cells and primary rat hepatocytes

Journal of Hepatology
Axel M GressnerB Polzar

Abstract

Primary cultures of rat hepatocytes, rat (FAO) and human (HepG2) hepatoma cells were studied by immunocytochemistry for expression of transforming growth factor (TGF)-beta, for the release of TGF-beta into the medium, and generation of hepatocellular apoptosis by the respective cell-conditioned media. Using the alkaline-phosphatase anti-alkaline-phosphatase technique, intense TGF-beta immunostaining was shown in all cell types. The cytokine is released almost entirely in the latent form into the culture medium; only the FAO-cells had a substantial fraction of bioactive TGF-beta in the native (unacidified) culture fluid. Exposure of hepatocytes with the respective cell-conditioned media in the activated, but not in the native form (except for FAO-cell media), induced severe detrimental effects as evidenced by: (i) gross morphological alterations, (ii) functional impairment (reduction of WST-1 test, detachment of cells, lactate dehydrogenase increase in the medium), and (iii) generation of apoptosis. The latter phenomenon was confirmed by an increase of internucleosomal DNA fragments, positive TUNEL reaction, and intense binding of the fluorochrome Hoechst 33342 to fragmented nuclei. All these effects, which were mimicked by addi...Continue Reading

References

Nov 1, 1992·The Journal of Cell Biology·Y GavrieliS A Ben-Sasson
Feb 1, 1992·Molecular Biology of the Cell·J E Murphy-UllrichM Höök
Jun 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·F A OberhammerR Schulte-Hermann
Jan 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·P A Dennis, D B Rifkin
Apr 1, 1990·The Journal of Cell Biology·R M LyonsH L Moses
Mar 1, 1990·Japanese Journal of Cancer Research : Gann·N ItoS Tarui
Aug 31, 1990·Biochemical and Biophysical Research Communications·K TakaishiS Tarui
Mar 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·L BraunN Fausto
Nov 1, 1986·Journal of Clinical Chemistry and Clinical Biochemistry. Zeitschrift Für Klinische Chemie Und Klinische Biochemie·A M Gressner, T Pfeiffer
Mar 1, 1980·Analytical Biochemistry·C Labarca, K Paigen
Feb 1, 1984·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·J L CordellD Y Mason
Oct 1, 1995·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·G KroemerB Mignotte
Nov 1, 1995·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·A BenedettiA M Jezequel
May 1, 1995·Cell Biology International·D A Cox
Jan 1, 1993·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·J H WijsmanJ H van Dierendonck
Oct 1, 1993·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·K FukudaJ F Chiu
Mar 28, 1995·Proceedings of the National Academy of Sciences of the United States of America·N SandersonS S Thorgeirsson
May 1, 1995·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·B Grasl-KrauppR Schulte-Hermann
Mar 1, 1995·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·P BedossaT Poynard
Oct 1, 1994·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·J R HullyN A Gillett
Nov 10, 1994·The New England Journal of Medicine·W A Border, N A Noble
Apr 1, 1994·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·H EnzanH Hara
May 1, 1994·Gut·M R Alison, C E Sarraf
Nov 1, 1993·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·F OberhammerR Schulte-Hermann
Aug 1, 1993·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·R H SchwallT G Terrell
Mar 1, 1993·British Journal of Cancer·W BurschR Schulte-Hermann
Mar 1, 1996·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·M YamamotoY Komatsu
May 31, 2011·Current Pharmaceutical Biotechnology·Andreas Hilbig, Helmut Oettle

❮ Previous
Next ❯

Citations

Jul 10, 1998·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·H C Pitot
Jan 29, 2000·Liver Transplantation : Official Publication of the American Association for the Study of Liver Diseases and the International Liver Transplantation Society·G J Gores
Sep 2, 2008·Archives of Dermatological Research·Fukumi FurukawaYoshiki Miyachi
May 24, 2003·Journal of Hepatology·Ulrike BöerGerhard P Püschel
Aug 9, 2003·European Journal of Pharmaceutical Sciences : Official Journal of the European Federation for Pharmaceutical Sciences·C C ChouJ H Guh
May 29, 2008·Journal of Gastroenterology and Hepatology·Olav A GressnerAxel M Gressner
Aug 17, 1999·Annual Review of Nutrition·M LipkinS A Lamprecht
May 4, 2007·Comparative Hepatology·Lucia WickertAxel M Gressner
Aug 1, 2007·Comparative Hepatology·Olav A GressnerAxel M Gressner
Mar 23, 2005·Liver International : Official Journal of the International Association for the Study of the Liver·Toshiaki MoriAkimasa Nakao
Apr 23, 2004·Liver International : Official Journal of the International Association for the Study of the Liver·Jens HerrmannRalf Weiskirchen
Jun 16, 2012·Liver International : Official Journal of the International Association for the Study of the Liver·Erawan Borkham-KamphorstRalf Weiskirchen
Apr 5, 2011·Liver International : Official Journal of the International Association for the Study of the Liver·Erawan Borkham-KamphorstRalf Weiskirchen
Jul 25, 2014·European Journal of Pharmacology·Leila SafaeianGolnaz Vaseghi
Mar 13, 2014·Clinica Chimica Acta; International Journal of Clinical Chemistry·Olav A Gressner, Chunfang Gao
Oct 11, 2008·Journal of Hepatology·Irene Carmona-CuencaIsabel Fabregat
Sep 19, 1998·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·S AngermüllerG Tiegs
Jul 13, 2000·Human & Experimental Toxicology·V GollL Richert
Feb 3, 2004·Journal of Cellular Physiology·Nikolaus BresgenP M Eckl
Sep 22, 1999·Oncogene·M C YakicierM Ozturk
Apr 1, 2017·American Journal of Physiology. Lung Cellular and Molecular Physiology·Qiang DingVictor J Thannickal
Sep 19, 2002·Glia·Norbert SchusterKerstin Krieglstein
Nov 26, 1999·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·A RibeiroG J Gores
Sep 16, 2004·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·María de Luján AlvarezMaría Cristina Carrillo
Jul 9, 2009·Journal of Cellular and Molecular Medicine·Fraczek JoannaRogiers Vera
Jul 18, 2009·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Olav A Gressner
Feb 15, 2020·Journal of Receptor and Signal Transduction Research·Bhagyalakshmi Nair, Lekshmi R Nath
Feb 1, 2003·European Journal of Gastroenterology & Hepatology·Robin D Hughes, Lee W Evans
Jun 11, 2002·The Journal of Immunology : Official Journal of the American Association of Immunologists·Naoki HagimotoNobuyuki Hara
Jul 31, 2003·The Journal of Nutrition·Hélène MartinAlain Berthelot
Sep 23, 2003·European Journal of Gastroenterology & Hepatology·Murat KiyiciFaruk Memik

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.