TGF-beta1 induces morphogenesis of branching cords by cloned mammary epithelial cells at subpicomolar concentrations

Biochemical and Biophysical Research Communications
J V SorianoR Montesano

Abstract

The factors which control the formation of lactiferous ducts during mammary gland development are not yet fully understood. To address this issue, we assessed the effect of the multifunctional cytokine transforming growth factor-beta-1 (TGF-beta1) on the morphogenetic properties of TAC-2.1 mammary epithelial cells grown in collagen gels. Here we demonstrate that TGF-beta1, has a biphasic effect on these cells: whereas relatively high concentrations (0.5-5 ng/ml) inhibit colony formation, lower concentrations (5-100 pg/ml = 200fM-4pM) stimulate colony elongation and branching, resulting in the formation of an extensive network of epithelial cords. We suggest that, in concert with other regulatory factors, low concentrations of TGF-beta1 promote mammary gland ductal morphogenesis.

Citations

Apr 20, 2005·Journal of Mammary Gland Biology and Neoplasia·Kenna R Mills ShawJoan S Brugge
Mar 20, 2012·Proceedings of the National Academy of Sciences of the United States of America·Chin-Lin GuoChih-Yu Shen
Sep 3, 2010·Cold Spring Harbor Perspectives in Biology·Harold Moses, Mary Helen Barcellos-Hoff
Aug 20, 2008·BMC Cell Biology·J M FlemingB K Vonderhaar
Sep 1, 2006·Biochemical and Biophysical Research Communications·Susanne Wurm, Christian Wechselberger
Jul 16, 2008·Biochemical and Biophysical Research Communications·Roberto MontesanoHerbert Schramek
Dec 30, 2016·The Journal of Cell Biology·Marina Simian, Mina J Bissell
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