TGF-beta2 alters the characteristics of the neuromuscular junction by regulating presynaptic quantal size.

Proceedings of the National Academy of Sciences of the United States of America
Sitt Wai FongGuy S Bewick

Abstract

The amount of neurotransmitter released from a presynaptic terminal is the product of the quantal content (number of vesicles) and the presynaptic quantal size (QSpre, amount of transmitter per vesicle). QSpre varies with synaptic use, but its regulation is poorly understood. The motor nerve terminals at the neuromuscular junction (NMJ) contain TGF-beta receptors. We present evidence that TGF-beta2 regulates QSpre at the NMJ. Application of TGF-beta2 to the rat diaphragm NMJ increased the postsynaptic response to both spontaneous and evoked release of acetylcholine, whereas antibodies to TGF-beta2 or its receptor had the converse effect. L-vesamicol and bafilomycin blocked the actions of TGF-beta2, indicating that TGF-beta2 acts by altering the extent of vesicular filling. Recordings of the postsynaptic currents from the diaphragm were consistent with TGF-beta2 having this presynaptic action and a lesser postsynaptic effect on input resistance. TGF-beta2 also decreased quantal content by an atropine-sensitive pathway, indicating that this change is secondary to cholinergic feedback on vesicular release. Consequently, the net actions of TGF-beta2 at the NMJ were to amplify the postsynaptic effects of spontaneous transmission and...Continue Reading

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Citations

Mar 11, 2011·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Xueyong WangKathrin L Engisch
Aug 10, 2016·Journal of Cell Science·Eleni RoussaKerstin Krieglstein
Apr 22, 2011·Developmental Neurobiology·Neil Dani, Kendal Broadie
Nov 1, 2018·Molecular Neurobiology·Luan Pereira DinizFlávia Carvalho Alcantara Gomes
Mar 20, 2018·Scientific Reports·Marco MorschWilliam D Phillips
Aug 28, 2020·Trends in Neurosciences·Anastasia Gromova, Albert R La Spada
May 17, 2011·Life Sciences·Joice StipurskyFlávia Carvalho Alcantara Gomes
Apr 6, 2021·Aging and Disease·Sandra Fuertes-Alvarez, Ander Izeta

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