TGFβ1-induced expression of caldesmon mediates epithelial-mesenchymal transition

Cytoskeleton
Sandeep M NalluriEsther W Gomez

Abstract

Epithelial-mesenchymal transition (EMT) is an important process that mediates organ development and wound healing, and in pathological contexts, it can contribute to the progression of fibrosis and cancer. During EMT, cells exhibit marked changes in cytoskeletal organization and increased expression of a variety of actin associated proteins. Here, we sought to determine the role of caldesmon in mediating EMT in response to transforming growth factor (TGF)-β1. We find that the expression level and phosphorylation state of caldesmon increase as a function of time following induction of EMT by TGFβ1 and these changes in caldesmon correlate with increased focal adhesion number and size and increased cell contractility. Knockdown and forced expression of caldesmon in epithelial cells reveals that caldesmon expression plays an important role in regulating the expression of the myofibroblast marker alpha smooth muscle actin. Results from these studies provide insight into the role of cytoskeletal associated proteins in the regulation of EMT and may suggest ways to target the cell cytoskeleton for regulating EMT processes.

References

Nov 28, 1971·Journal of Molecular Biology·S Fujime, S Ishiwata
Jan 1, 1995·Cell Motility and the Cytoskeleton·I Surgucheva, J Bryan
Jun 1, 1996·The Journal of Cell Biology·M Chrzanowska-Wodnicka, K Burridge
Feb 12, 1998·Proceedings of the National Academy of Sciences of the United States of America·R J Pelham, Y l Wang
Mar 30, 1999·Biophysical Journal·M Dembo, Y L Wang
Oct 8, 1999·Molecular Biology of the Cell·D M HelfmanA D Bershadsky
Sep 13, 2001·Molecular Biology of the Cell·B HinzC Chaponnier
May 4, 2002·Nature Reviews. Molecular Cell Biology·James J TomasekRobert A Brown
Aug 15, 2002·Current Biology : CB·John A Cooper
Dec 12, 2002·Chest·Sem H Phan
Dec 31, 2002·American Journal of Physiology. Renal Physiology·Andras MassziAndrás Kapus
Apr 3, 2003·International Journal of Cancer. Journal International Du Cancer·Christèle BissonAgnès Noel
Jun 17, 2003·Molecular Biology of the Cell·Boris HinzChristine Chaponnier
Jan 31, 2004·Journal of Molecular Medicine : Official Organ of the Gesellschaft Deutscher Naturforscher Und Ärzte·Michael Zeisberg, Raghu Kalluri
Apr 16, 2004·Breast Cancer Research : BCR·Kimberly A BrownHarold L Moses
Oct 1, 2004·The Journal of Biological Chemistry·D Brian FosterC-L Albert Wang
Nov 3, 2004·Journal of Cellular Physiology·Tamara MirzapoiazovaAlexander D Verin
Nov 19, 2004·Neoplasia : an International Journal for Oncology Research·Lu XieHarold L Moses
Apr 12, 2005·Annual Review of Pharmacology and Toxicology·R Dale BrownCarlin S Long
Sep 24, 2005·Journal of Cell Science·Derek C Radisky
Jan 13, 2006·The Journal of Cell Biology·Jérôme M GoffinBoris Hinz
Jan 13, 2006·Kidney International·Youhua Liu
Feb 3, 2006·Journal of Biomedical Science·Jolanta KordowskaChih-Lueh Albert Wang
Mar 28, 2006·Journal of Cellular and Molecular Medicine·A M Gressner, R Weiskirchen
Apr 6, 2006·Journal of Cell Science·Robert EvesAlan S Mak
May 9, 2006·Experimental Eye Research·Inna GroshevaAlexander D Bershadsky
Jun 2, 2006·Proceedings of the American Thoracic Society·Brigham C WillisZea Borok
Aug 8, 2006·Tissue Engineering·Daniël T LuttikhuizenMarja J A Van Luyn
Aug 9, 2006·Journal of Biomaterials Science. Polymer Edition·Christina GretzerPeter Thomsen
Dec 22, 2006·Cell Motility and the Cytoskeleton·Jianxin ChenJames H-C Wang
Feb 14, 2007·Journal of Biomedical Materials Research. Part a·Allen G LiW Kenneth Ward
Feb 15, 2007·The Journal of Investigative Dermatology·Boris Hinz

❮ Previous
Next ❯

Citations

Aug 15, 2019·Annual Review of Cell and Developmental Biology·Michael Sheetz

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.