TGFβ2-mediated production of hyaluronan is important for the induction of epicardial cell differentiation and invasion.

Experimental Cell Research
Evisabel CraigTodd D Camenisch

Abstract

In the developing heart, the epicardium is a major source of progenitor cells that contribute to the formation of the coronary vessel system. These epicardial progenitors give rise to the different cellular components of the coronary vasculature by undergoing a number of morphological and physiological changes collectively known as epithelial to mesenchymal transformation (EMT). However, the specific signaling mechanisms that regulate epicardial EMT are yet to be delineated. In this study we investigated the role of TGFβ2 and hyaluronan (HA) during epicardial EMT and how signals from these two molecules are integrated during this important process. Here we show that TGFβ2 induces MEKK3 activation, which in turn promotes ERK1/2 and ERK5 phosphorylation. TGFβ2 also increases Has2 expression and subsequent HA production. Nevertheless, inhibition of MEKK3 kinase activity, silencing of ERK5 or pharmacological disruption of ERK1/2 activation significantly abrogates this response. Thus, TGFβ2 promotes Has2 expression and HA production through a MEKK3/ERK1/2/5-dependent cascade. Furthermore, TGFβ2 is able to induce epicardial cell invasion and differentiation but not proliferation. However, inhibition of MEKK3-dependent pathways, degra...Continue Reading

References

Oct 23, 1997·Developmental Dynamics : an Official Publication of the American Association of Anatomists·J M Pérez-PomaresR Muñoz-Chápuli
Jan 27, 1998·The Journal of Biological Chemistry·A P Spicer, J A McDonald
Jul 3, 2002·Proceedings of the National Academy of Sciences of the United States of America·Christopher P ReganKeisuke Kuida
Jan 7, 2003·IUBMB Life·Naoki Itano, Koji Kimata
Jun 20, 2003·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Daniël G M MolinAdriana C Gittenberger-de-Groot
Jun 27, 2003·Journal of Molecular Medicine : Official Organ of the Gesellschaft Deutscher Naturforscher Und Ärzte·Joyce A SchroederTodd D Camenisch
Nov 25, 2003·Nature Cell Biology·Mark T UhlikGary L Johnson
Apr 1, 2004·Birth Defects Research. Part C, Embryo Today : Reviews·Andrew P Spicer, Janet Y L Tien
Nov 6, 2004·Circulation Research·Daniel G M MolinAdriana C Gittenberger-de Groot
Jul 16, 2005·Current Eye Research·Takahisa KogaHidenobu Tanihara
Nov 1, 2005·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Leigh A ComptonJoey V Barnett
Jan 13, 2006·The Journal of Biological Chemistry·Anne FritzRichard R Vaillancourt
Aug 18, 2006·Development·José Rivera-FelicianoWilliam T Pu
Nov 6, 2007·Biochimica Et Biophysica Acta·A BerdiakiG N Tzanakakis
Jan 24, 2008·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Anita F AustinJoey V Barnett
Oct 14, 2008·Experimental Biology and Medicine·Joseph T RawlinsLynne A Opperman
Oct 16, 2008·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Evisabel A CraigTodd D Camenisch
May 20, 2009·Glycobiology·Evisabel A CraigTodd D Camenisch
Aug 8, 2009·Circulation Research·Michelle D Combs, Katherine E Yutzey
Sep 10, 2009·The Journal of Biological Chemistry·Davide VigettiAlberto Passi
Sep 12, 2009·Experimental & Molecular Medicine·Ji-Ung JungYoung-Kug Choo

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Citations

Jun 14, 2014·Journal of Developmental Biology·Marie M LockhartAndy Wessels
Aug 26, 2014·Toxicological Sciences : an Official Journal of the Society of Toxicology·Tianfang HuangTodd D Camenisch
Jun 9, 2012·Circulation Research·Alexander von Gise, William T Pu
Dec 22, 2011·Future Cardiology·Nicola Smart, Paul R Riley
Dec 10, 2015·Cell Adhesion & Migration·Patrick AllisonTodd D Camenisch
Oct 9, 2015·International Journal of Cell Biology·Shibnath GhatakSuniti Misra
Jun 3, 2011·Current Opinion in Cell Biology·Elliott J Hagedorn, David R Sherwood
Jun 21, 2014·Journal of the American Heart Association·Alyssa A ChamberlainBin Zhou
Oct 25, 2014·Journal of the American Heart Association·Cristi L GalindoJohn H Cleator
Mar 19, 2019·Journal of Anatomy·Chrysostomos PerdiosSiobhan Loughna
Jan 31, 2020·Circulation Research·Pearl QuijadaEric M Small
Aug 28, 2019·Cold Spring Harbor Perspectives in Biology·Yingxi CaoJingli Cao
May 28, 2011·Birth Defects Research. Part A, Clinical and Molecular Teratology·Marie LockhartAndy Wessels
May 17, 2017·Frontiers in Cell and Developmental Biology·Angel DueñasDiego Franco
May 1, 2021·Journal of Cardiovascular Development and Disease·Marietta EasterlingMichael Bressan

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