PMID: 10598907Dec 22, 1999

TGFbeta isoforms and decorin gene expression are modified in fibroblasts obtained from non-syndromic cleft lip and palate subjects

Journal of Dental Research
Maria BodoPaolo Carinci

Abstract

Interaction between extracellular matrix (ECM) and cytokines is thought to be crucial for palatal development. The localization of transforming growth factors (TGFalpha and TGFbeta isoforms) in craniofacial tissues suggests that they carry out multiple functions during development. In the present report, we studied TGFalpha, TGFbeta1, and TGFbeta3 expressions and their effects on ECM macromolecule production of normal and cleft palatal fibroblasts in vitro, to investigate the mechanisms by which the phenotypic modulation of fibroblasts occurs during the cleft palate process. The results indicated that, while TGFalpha mRNA was not evidenced in CLP or normal fibroblasts, a reduced TGFbeta1 hybridization signal was detected in CLP fibroblasts. In addition, these secreted more active TGFbeta3 than TGFbeta1, as evaluated in a biological assay. The CLP phenotype, which differed from the normal one because of its higher PG decorin expression and greater production of GAG and collagen, was further modified by the addition of growth factors. In fact, in CLP fibroblasts, TGFalpha and TGFbeta1 down-regulated PG decorin transcript, TGFbeta1 increased collagen and GAG in both cellular and extracellular compartments, and TGFbeta3 promoted se...Continue Reading

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Citations

Aug 26, 2011·Journal of Human Genetics·Iman SalahshourifarBin Alwi Zilfalil
Sep 12, 2007·Archives of Medical Research·Giordano StabelliniF Pezzetti
Jul 25, 2008·Journal of Dental Research·J LiuJohannes W Von den Hoff
Mar 7, 2003·The Journal of Craniofacial Surgery·Francesco CarinciMauro Tognon
Jul 2, 2010·The Cleft Palate-craniofacial Journal : Official Publication of the American Cleft Palate-Craniofacial Association·Nicoletta GaglianoGiordano Stabellini
Aug 26, 2018·Oral Diseases·Mohammad Faisal J KhanMichele Rubini
Jan 9, 2008·Birth Defects Research. Part A, Clinical and Molecular Teratology·Diana M Juriloff, Muriel J Harris
Nov 13, 2007·The Journal of Craniofacial Surgery·Rosario RulloFrancesco Carinci
Sep 28, 2002·Journal of Dental Research·K KohamaMasamichi Ohishi
Oct 28, 2019·Genes·Aysel MammadovaJohannes W Von den Hoff
Nov 23, 2018·International Journal of Molecular Sciences·Akira NakajimaJun-Ichi Hanai

Related Concepts

DCN protein, human
Cleft Lip
Cleft Palate, Isolated
Zyderm
Fibroblasts
Glycosaminoglycans
Structure of Papilla Incisiva of Mouth
Proteoglycan Type H
Poly(A) Tail
Receptor Down-Regulation

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