Abstract
Type 1 transforming growth factor β receptor (TGFBR1)*6A, a common hypomorphic variant of TGFBR1, may act as a susceptibility allele in colorectal cancer. However, the contribution of TGFBR1*6A to colorectal cancer development is largely unknown. To test the hypothesis that TGFBR1*6A promotes colorectal cancer invasion and metastasis via Smad-independent transforming growth factor-β (TGF-β) signaling, the effect of TGFBR1*6A on the invasion of colorectal cancer cells was assessed. pCMV5-TGFBR1*6A-HA plasmids were transfected into SW48 and DLD-1 cells by Lipofectamine-mediated DNA transfection. The effect of TGF-β1 on the proliferation of SW48 and DLD-1 cells transfected with TGFBR1*6A was determined by MTT assay. The effects of the TGF-β1 on the invasion of the transfected SW48 and DLD-1 cells were determined using Matrigel-coated plates. Transforming migrating chambers were used to determine the effects of TGF-β1 on the migration of the transfected SW48 and DLD-1 cells. Western blot analysis was used to determine the expression of phosphorylated (p-) extracellular-signal-regulated kinase (ERK), p-P38 and p-SMAD family member 2 in SW48 cells. Using transfected TGFBR1*6A SW48 and DLD-1 cell lines our group demonstrated that, in ...Continue Reading
References
Feb 18, 2004·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Boris PascheTongzhang Zheng
Oct 6, 2005·JAMA : the Journal of the American Medical Association·Boris PascheChristopher M Weghorst
Mar 5, 2008·Cancer Research·Diana S RosmanBoris Pasche
Jun 23, 2009·BMC Cancer·Adela CastillejoJosé-Luís Soto
May 1, 2010·BMC Cancer·Yun-Sheng HuBao-An Ma
Aug 23, 2011·Molecular Biology Reports·Xueli ZhangMing Zhang
Aug 21, 2012·PloS One·Yong-qiang WangQiao-nan Guo
Aug 23, 2012·Molecular Carcinogenesis·Jianjian ChenHongbing Shen
Nov 10, 2012·Cancer Research·Nicholas I FlemingOliver M Sieber
Jan 5, 2013·Critical Reviews in Oncology/hematology·William K K WuJun Yu
Feb 11, 2014·Journal of the National Cancer Institute·Daniel R PrincipePaul J Grippo
Mar 29, 2014·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Ganesan Ramamoorthi, Nageswaran Sivalingam
Jun 2, 2014·Journal of Gastrointestinal Cancer·Tony IbrahimGeorges Chahine
Jul 2, 2014·BMC Cancer·Jason P RossGarry N Hannan
Jun 4, 2015·Reproduction, Fertility, and Development·Xiao-Feng SunXi-Feng Zhang
Oct 17, 2015·Brain, Behavior, and Immunity·Sarah TavesRu-Rong Ji
Feb 24, 2016·Cell·Charles J DavidJoan Massagué
Oct 25, 2016·Gastroenterology·Barbara JungDaniel Beauchamp
Nov 29, 2016·Oncogene·W-T LiuH-B Huang
Mar 8, 2017·PloS One·Amir Mehrvarz SarshekehScott Kopetz
May 26, 2017·PloS One·UNKNOWN PLOS ONE Staff