TH-17 cells in rheumatoid arthritis.

Arthritis Research & Therapy
Shiva ShahraraRichard M Pope

Abstract

The aim of this study was to quantify the number of T-helper (TH)-17 cells present in rheumatoid arthritis (RA) synovial fluid (SF) and to determine the level of interleukin (IL)-17 cytokine in RA, osteoarthritis (OA) and normal synovial tissue, as well as to examine SF macrophages for the presence of IL-23, IL-27 and interferon (IFN)-gamma. Peripheral blood (PB) mononuclear cells from normal and RA donors and mononuclear cells from RA SF were examined either without stimulation or after pretreatment with IL-23 followed by stimulation with phorbol myristate acetate (PMA) plus ionomycin (P/I). The abundance of TH-17 cells in RA SF was determined by flow cytometry. IL-17 levels were quantified in synovial tissue from RA, OA and normal individuals by ELISA and IL-23 was identified in SFs by ELISA. RA SF and control in vitro differentiated macrophages were either untreated or treated with the toll-like receptor (TLR) 2 ligand peptidoglycan, and then IL-23, IL-27 and IFN-gamma mRNA levels were quantified by real-time polymerase chain reaction (RT-PCR). Treatment with P/I alone or combined with IL-23 significantly increased the number of TH-17 cells in normal, RA PB and RA SF. With or without P/I plus IL-23, the percentage of TH-17 c...Continue Reading

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Citations

Sep 28, 2010·Archivum Immunologiae Et Therapiae Experimentalis·Marek JankowskiAnna Goc
Aug 13, 2011·Rheumatology International·Ali TaylanNurullah Akkoc
Dec 25, 2010·Clinical Rheumatology·Amit Kumar SinghAmita Aggarwal
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Feb 18, 2010·Arthritis Research & Therapy·Theo S PlantingaLeo Ab Joosten
Nov 8, 2011·Bioscience, Biotechnology, and Biochemistry·Hyun-Ju IhnKwang Dong Kim
Aug 8, 2012·PloS One·Irene Arroyo-VillaMaría-Eugenia Miranda-Carús
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Jul 12, 2012·Expert Opinion on Biological Therapy·Jangwon HeoQuan Dong Nguyen
Mar 22, 2016·Mediators of Inflammation·Michal A Rahat, Jivan Shakya
Dec 22, 2012·Archives of Biochemistry and Biophysics·Qi-Quan Huang, Richard M Pope

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Methods Mentioned

BETA
PMA
ELISA
PCR

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