TH1 cell-inducing Escherichia coli strain identified from the small intestinal mucosa of patients with Crohn's disease.

Gut Microbes
Manabu NagayamaKenya Honda

Abstract

Dysbiotic microbiota contributes to the pathogenesis of Crohn's disease (CD) by regulating the immune system. Although pro-inflammatory microbes are probably enriched in the small intestinal (SI) mucosa, most studies have focused on fecal microbiota. This study aimed to examine jejunal and ileal mucosal specimens from patients with CD via double-balloon enteroscopy. Comparative microbiome analysis revealed that the microbiota composition of CD SI mucosa differs from that of non-CD controls, with an increased population of several families, including Enterobacteriaceae, Ruminococcaceae, and Bacteroidaceae. Upon anaerobic culturing of the CD SI mucosa, 80 bacterial strains were isolated, from which 9 strains representing 9 distinct species (Escherichia coli, Ruminococcus gnavus, Klebsiella pneumoniae, Erysipelatoclostridium ramosum, Bacteroides dorei, B. fragilis, B. uniformis, Parabacteroides distasonis, and Streptococcus pasteurianus) were selected on the basis of their significant association with CD. The colonization of germ-free (GF) mice with the 9 strains enhanced the accumulation of TH1 cells and, to a lesser extent, TH17 cells in the intestine, among which an E. coli strain displayed high potential to induce TH1 cells an...Continue Reading

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Citations

Dec 2, 2020·Trends in Microbiology·Fernanda Pace, Paula I Watnick
Jul 12, 2021·Cellular and Molecular Gastroenterology and Hepatology·Ting-Jia FanJonathan J Hansen

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Methods Mentioned

BETA
biopsy
scraping
flow cytometry
biopsies
PMA

Software Mentioned

PERMANOVA
Flowjo
UCLUST
join
fastq
phyloseq
GLSEARCH
R
GraphPad Prism

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