Th1 epitope repertoire on the alpha subunit of human muscle acetylcholine receptor in myasthenia gravis

Neurology
Z Y WangB M Conti-Fine

Abstract

In myasthenia gravis (MG), CD4+ T helper cells recognize the muscle acetylcholine receptor (AChR) alpha subunit. We investigated the epitope repertoire of anti-AChR blood CD4+ Th1 cells from 13 myasthenic patients and three healthy controls, using overlapping synthetic peptides screening the alpha subunit sequence and an enzyme-linked immunospot (ELISPOT) assay that detects antigen-induced interferon-gamma secretion of individual Th1 cells. All patients recognized a pool of the alpha subunit peptides. All but one patient recognized numerous peptides. Each patient had an individual pattern of peptide recognition, but most or all patients recognized four sequences (residues 48-67, 101-137, 304-322, and 403-437) that stimulated relatively large numbers of Th1 cells. They include previously identified "immunodominant" sequences recognized by AChR-specific CD4+ T cell lines from myasthenic patients. Peptide 1-14 was also recognized frequently. The controls recognized, with a low precursor frequency, the peptide pool and a few peptides that frequently included the immunodominant sequences described above. The present results demonstrate that Th1 cells are involved in the anti-AChR response in MG and that their epitope repertoire is v...Continue Reading

Citations

Mar 15, 2000·Muscle & Nerve·J M Lindstrom
Aug 12, 1999·Annals of Neurology·A SchultzA Marx
Sep 16, 2010·Current Treatment Options in Neurology·Agnes Jani-Acsadi, Robert P Lisak
Jul 20, 2000·Journal of Neuroimmunology·Z Y WangB M Conti-Fine
May 29, 2000·Immunology Today·B BalasaN Sarvetnick
Mar 14, 2009·Nature Protocols·Hendrik StreeckBruce D Walker
Jul 21, 1998·Annals of the New York Academy of Sciences·B M Conti-FineZ Y Wang
May 22, 2002·Journal of Neuroimmunology·Sin Hyeog ImSara Fuchs
Oct 3, 2003·Journal of Thrombosis and Haemostasis : JTH·G-L HuB M Conti-Fine
Aug 13, 2003·Journal of Thrombosis and Haemostasis : JTH·M T RedingB M Conti-Fine
Nov 1, 2003·Annals of the New York Academy of Sciences·Monica MilaniBianca M Conti-Fine
Nov 25, 2006·Haemophilia : the Official Journal of the World Federation of Hemophilia·M T Reding
Jun 24, 2008·Annals of the New York Academy of Sciences·Bianca M Conti-FineWei Wang
Sep 2, 2006·Journal of Neuroimmunology·Monica MilaniBianca M Conti-Fine
Jan 8, 1999·Journal of Autoimmunity·D NavaneethamB M Conti-Fine
Apr 17, 2002·The Journal of Clinical Investigation·Huan YangPremkumar Christadoss
Aug 12, 2004·Journal of Thrombosis and Haemostasis : JTH·M T RedingB M Conti-Fine
May 9, 2000·The Journal of Immunology : Official Journal of the American Association of Immunologists·P I KarachunskiB M Conti-Fine
Apr 6, 2001·The Journal of Immunology : Official Journal of the American Association of Immunologists·N S OstlieB M Conti-Fine
Nov 22, 2002·The Journal of Immunology : Official Journal of the American Association of Immunologists·Sue StacyEllen Kraig
Jul 3, 2018·Frontiers in Immunology·Sandeep Kumar DhandaAlessandro Sette

❮ Previous
Next ❯

Related Concepts

Related Feeds

Autoimmune Diseases

Autoimmune diseases occur as a result of an attack by the immune system on the body’s own tissues resulting in damage and dysfunction. There are different types of autoimmune diseases, in which there is a complex and unknown interaction between genetics and the environment. Discover the latest research on autoimmune diseases here.