Th17 responses are not induced in dextran sodium sulfate model of acute colitis.

Immune Network
Yoon Suk KimKi-Jong Rhee

Abstract

Dextran sodium sulfate (DSS) is a widely used chemical model for inflammatory bowel disease (IBD). It is thought that imbalances in the T helper (Th) cell subsets contribute to IBD. Recent studies suggest that the acute DSS-colitis model is polarized toward a Th1/Th17 profile based on RT-PCR analysis of colonic tissues. In the current study we determined whether colonic Th cells from DSS-colitis mice were skewed toward the Th17 profile. Mice were treated with 5% DSS for 7 days and colonic T cells isolated and examined for production of IFN-γ (Th1 cell), IL-4 (Th2 cell) and IL-17 (Th17 cell) by intracellular flow cytometry. We found that the percentage of colonic Th17 cells were similar to non-treated controls but the percentage of Th1 cells were elevated in DSS-colitis mice. These results suggest that in the acute DSS-colitis model the colonic Th cells exhibit a Th1 profile and not a Th17 profile.

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Citations

Jun 21, 2014·Mediators of Inflammation·Seok-Jae KoJae-Woo Park
Apr 9, 2013·Pharmacology & Therapeutics·Giovanni DothelFabrizio De Ponti
Aug 18, 2016·International Immunopharmacology·Lixuan SangDanan Wang
Oct 21, 2016·Inflammatory Bowel Diseases·Rocío López-PosadasFermín Sánchez de Medina
May 10, 2016·World Journal of Gastrointestinal Pharmacology and Therapeutics·Satoshi InoKazuo Tanaka
Jan 7, 2015·World Journal of Gastroenterology : WJG·Fabiany da Costa GonçalvesAna Helena Paz
Mar 30, 2020·Neurogastroenterology and Motility : the Official Journal of the European Gastrointestinal Motility Society·Maria Francesca Viola, Guy Boeckxstaens
Sep 18, 2012·The Journal of Immunology : Official Journal of the American Association of Immunologists·Khalid W KalimMarcus Groettrup

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Methods Mentioned

BETA
flow cytometry
PMA
PCR

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