Abstract
It is widely considered that endoplasmic reticulum stress may rapidly induce apoptosis. The aim of the present study was to investigate the effect of thapsigargin on the induction of apoptosis in prostate cancer cells, and to explore its possible mechanism. A Cell Counting Kit-8 was selected to determine the effect of thapsigargin (0, 1, 10 and 100 nM) on the proliferation of PC3 cells. Cell proliferation of the prostate cancer cells was effectively inhibited by treatment with thapsigargin, and thapsigargin significantly increased the rate of apoptosis and caspase-3/9 activities in prostate cancer cells. The protein expression of phosphorylated (p)-RAC-α serine threonine-protein kinase, p-mechanistic target of rapamycin, F-actin and paxillin were significantly decreased, and cofilin-1 protein expression was significantly increased by treatment with thapsigargin in prostate cancer cells. Overall, the data of the present study revealed that thapsigargin induced apoptosis in prostate cancer cells through cofilin-1 and paxillin.
References
Jul 12, 2005·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Kirk H M YipJiake Xu
Sep 5, 2006·Cancer Research·Beibei ZhuNatasha Kyprianou
Mar 9, 2007·European Journal of Pharmacology·Yuko MuramatsuNobuya Matsuoka
Jan 9, 2009·PloS One·Hannes C A Drexler
Nov 3, 2010·Oncology Reports·Sivan M BokobzaWen G Jiang
May 5, 2011·Urology·Evan Y YuKarim Fizazi
Apr 24, 2012·PloS One·Vasudha SundramMeena Jaggi
Feb 16, 2013·Diabetologia·S AsaharaY Kido
Mar 2, 2013·Annals of Oncology : Official Journal of the European Society for Medical Oncology·B M WolpinM Hidalgo
Dec 10, 2013·Oncology Reports·Yong LuoLing Lan
Feb 25, 2014·Magnetic Resonance Imaging·Andriy FedorovFiona M Fennessy
Mar 13, 2014·Prostate Cancer and Prostatic Diseases·R ThomasP Bellamy
May 2, 2014·Medical Oncology·Kazuhiro MatsumotoSo Nakamura
Sep 4, 2014·Methods in Molecular Biology·Stephen R HammesAritro Sen
Sep 13, 2014·The Prostate·Pinar ObakanNarcin Palavan-Unsal
Oct 31, 2014·The Oncologist·Nadine HouédéPierre Vincenzo Piazza
Nov 2, 2014·Annals of Oncology : Official Journal of the European Society for Medical Oncology·D CellaK Fizazi
Feb 11, 2015·Oncology Letters·Fang FangLiguo Wang
Apr 3, 2015·The Prostate·Yue YuXuesen Dong
May 7, 2015·PloS One·Ling-Yen ChiuGwo-Tarng Sheu
Jul 3, 2015·Oncology Letters·L I LuXiao-Ping Li
Jul 22, 2015·Oncotarget·Andrew K Kwegyir-AffulVincent C O Njar
Aug 13, 2015·Neurotoxicology·Adchara JanyouJiraporn Tocharus
Sep 24, 2015·JAMA : the Journal of the American Medical Association·Anthony V D'AmicoPhilip W Kantoff
Dec 30, 2015·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Minoru KatoMarianne D Sadar
Jan 26, 2016·Pharmacological Research : the Official Journal of the Italian Pharmacological Society·R S ReshmaPriya Srinivas
Jan 1, 2015·Annals of Oncology : Official Journal of the European Society for Medical Oncology·D CellaK Fizazi
Jul 1, 2013·Annals of Oncology : Official Journal of the European Society for Medical Oncology·B M WolpinM Hidalgo