Thawing boar semen in the presence of seminal plasma: Effects on sperm quality and fertility

Animal Reproduction Science
J C GarciaR N Kirkwood

Abstract

Causes of poor fertility after insemination of frozen-thawed (FT) sperm include reduced sperm viability at thawing and a shorter longevity of surviving sperm in the female genital tract due to sub-lethal damage. The present studies examined the effect of incubating thawed boar sperm in seminal plasma (SP) on sperm membrane integrity (viability), and motility in vitro (experiment 1), and fertility in vivo (experiment 2). For experiment 1, FT sperm from five individual boars and a sperm pool from these boars were thawed and incubated for 4 h in media containing 0%, 10%, or 50% autologous seminal plasma (individual boars) or pooled seminal plasma (sperm pool). At approximately 10 min (0 h) and again at 1 h, 2 h, 3 h, and 4 h, sperm populations were examined for percentage sperm viability and percent sperm motility. Each variable progressively decreased during the incubation period. Incubation in 50% SP increased percentages of live sperm (P < 0.0001) and percent sperm motility (P < 0.01) at all time points compared to incubation in either 0% or 10% SP. For experiment 2, multiparous Large white x Landrace sows (n = 82) each received 900 IU eCG at weaning and 750 IU hCG 80 h later to control time of ovulation. Sows were assigned on ...Continue Reading

References

Jan 1, 1990·Journal of Reproduction and Fertility·R A Harrison, S E Vickers
May 2, 2003·Biology of Reproduction·Fernando CenturionEmilio A Martinez
Jul 29, 2004·Reproduction : the Official Journal of the Society for the Study of Fertility·S O'LearyS A Robertson
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Citations

Oct 4, 2011·Reproduction : the Official Journal of the Society for the Study of Fertility·Tamara Leahy, Bart M Gadella
Mar 10, 2016·Reproductive Biology·Chung-Hsun YangJui-Te Wu
Feb 24, 2016·Evidence-based Complementary and Alternative Medicine : ECAM·Majid NaghdiNarges Fereydouni
Jul 10, 2012·Animal Reproduction Science·José Gómez-FernándezEduardo de Mercado
Dec 3, 2010·Reproduction in Domestic Animals = Zuchthygiene·A F C de AndradeR P Arruda
Sep 9, 2011·Reproduction in Domestic Animals = Zuchthygiene·T Leahy, B M Gadella
Jul 16, 2015·Reproduction in Domestic Animals = Zuchthygiene·M Yeste
Jul 16, 2015·Reproduction in Domestic Animals = Zuchthygiene·H Funahashi
Jul 16, 2015·Reproduction in Domestic Animals = Zuchthygiene·R V Knox
Jun 25, 2013·Computer Methods and Programs in Biomedicine·E AlegreL Sanchez
Jun 13, 2013·Theriogenology·Rocío Fernández-GagoFelipe Martínez-Pastor
Aug 22, 2016·Reproduction, Fertility, and Development·Rocío Fernández-GagoFelipe Martínez-Pastor
Jun 14, 2017·Molecular Reproduction and Development·Marc YesteSergi Bonet
Feb 22, 2021·Theriogenology·F Martínez-PastorJ M Morrell
Apr 4, 2021·Animals : an Open Access Journal From MDPI·Estíbaliz LacalleFelipe Martínez-Pastor
May 1, 2021·International Journal of Molecular Sciences·Heriberto Rodriguez-MartinezJordi Roca
Aug 18, 2021·Animal Reproduction Science·M WiebkeM Schulze

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