The γ-Aminobutyric Acid (GABA) Alleviates Salt Stress Damage during Seeds Germination of White Clover Associated with Na⁺/K⁺ Transportation, Dehydrins Accumulation, and Stress-Related Genes Expression in White Clover

International Journal of Molecular Sciences
Bizhen ChengYan Peng

Abstract

The objective of this study was to determine the effect of soaking with γ-aminobutyric acid (GABA) on white clover (Trifolium repens cv. Haifa) seed germination under salt stress induced by 100 mM NaCl. Seeds soaking with GABA (1 μM) significantly alleviated salt-induced decreases in endogenous GABA content, germination percentage, germination vigor, germination index, shoot and root length, fresh and dry weight, and root activity of seedling during seven days of germination. Exogenous application of GABA accelerated starch catabolism via the activation of amylase and also significantly reduced water-soluble carbohydrate, free amino acid, and free proline content in seedlings under salt stress. In addition, improved antioxidant enzyme activities (SOD, GPOX, CAT, APX, DHAR, GR and MDHR) and gene transcript levels (Cu/ZnSOD, FeSOD, MnSOD, CAT, GPOX, APX, MDHR, GPX and GST) was induced by seeds soaking with GABA, followed by decreases in O₂∙-, H₂O₂, and MDA accumulation during germination under salt stress. Seeds soaking with GABA could also significantly improve Na⁺/K⁺ content and transcript levels of genes encoding Na⁺/K⁺ transportation (HKT1, HKT8, HAL2, H⁺-ATPase and SOS1) in seedlings of white clover. Moreover, exogenous GABA...Continue Reading

References

Oct 26, 1999·Trends in Plant Science·B J ShelpM D McLean
Feb 14, 2002·Plant, Cell & Environment·R. Munns
Sep 16, 2003·Current Opinion in Plant Biology·Jian Kang Zhu
Mar 8, 2006·Journal of Experimental Botany·J CuarteroV Moreno
Nov 1, 1976·Plant Physiology·J F Tárrago, G Nicolás
Feb 1, 1977·Plant Physiology·C N Giannopolitis, S K Ries
Mar 23, 2010·Plant Physiology and Biochemistry : PPB·Jose M BarbosaRobert D Locy
Sep 8, 2011·Plant Signaling & Behavior·Moez HaninKhaled Masmoudi
May 23, 2012·Plant Science : an International Journal of Experimental Plant Biology·Cecilia RuibalSabina Vidal
Feb 21, 2014·Physiology and Molecular Biology of Plants : an International Journal of Functional Plant Biology·Parviz MalekzadehReza Heydari
Jan 26, 2017·Frontiers in Plant Science·Maria Grazia AnnunziataPetronia Carillo
Feb 20, 2018·Frontiers in Plant Science·Petronia Carillo

❮ Previous
Next ❯

Citations

May 8, 2020·Plant Signaling & Behavior·Ramakrishna Akula, Soumya Mukherjee
Dec 5, 2019·Metabolites·Sara González-OrengaMonica Boscaiu
Aug 8, 2020·Planta·Daniel Farias de OliveiraEnéas Gomes-Filho
Jan 7, 2021·Plant Signaling & Behavior·Li LiChunxia Wu
Apr 14, 2021·Scientific Reports·Puthanvila Surendrababu SwathyAnnamalai Muthusamy
Aug 11, 2021·The Plant Journal : for Cell and Molecular Biology·Wenjun LiZhoufei Wang

❮ Previous
Next ❯

Methods Mentioned

BETA
salt stress
MDA
environmental stresses
salt
transgenic
enzyme linked immunosorbent assay
PCR

Software Mentioned

SPSS

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.