The β-subunit of the SnRK1 complex is phosphorylated by the plant cell death suppressor Adi3.

Plant Physiology
Julian AvilaTimothy P Devarenne

Abstract

The protein kinase AvrPto-dependent Pto-interacting protein3 (Adi3) is a known suppressor of cell death, and loss of its function has been correlated with cell death induction during the tomato (Solanum lycopersicum) resistance response to its pathogen Pseudomonas syringae pv tomato. However, Adi3 downstream interactors that may play a role in cell death regulation have not been identified. We used a yeast two-hybrid screen to identify the plant SnRK1 (for Sucrose non-Fermenting-1-Related Protein Kinase1) protein as an Adi3-interacting protein. SnRK1 functions as a regulator of carbon metabolism and responses to biotic and abiotic stresses. SnRK1 exists in a heterotrimeric complex with a catalytic α-subunit (SnRK1), a substrate-interacting β-subunit, and a regulatory γ-subunit. Here, we show that Adi3 interacts with, but does not phosphorylate, the SnRK1 α-subunit. The ability of Adi3 to phosphorylate the four identified tomato β-subunits was also examined, and it was found that only the Galactose Metabolism83 (Gal83) β-subunit was phosphorylated by Adi3. This phosphorylation site on Gal83 was identified as serine-26 using a mutational approach and mass spectrometry. In vivo expression of Gal83 indicates that it contains multip...Continue Reading

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Citations

Dec 23, 2014·Journal of Plant Biology = Singmul Hakhoe Chi·Jen Sheen
Mar 20, 2013·Biochimica Et Biophysica Acta·Joel W GrayTimothy P Devarenne
Oct 27, 2011·Biochimica Et Biophysica Acta·Anna C Nelson Dittrich, Timothy P Devarenne
Mar 3, 2015·Frontiers in Plant Science·Sarah P WilliamsImara Y Perera
Jun 7, 2014·Frontiers in Plant Science·Pierre CrozetElena Baena-González
Feb 23, 2019·Journal of Experimental Botany·Leonor MargalhaElena Baena-González
Jul 22, 2017·Scientific Reports·Adil SalhiVladimir B Bajic
Jan 16, 2021·Journal of Experimental Botany·Jianwen SongZhibiao Ye
May 20, 2018·Biochimica Et Biophysica Acta. Proteins and Proteomics·Dongyin Su, Timothy P Devarenne
Mar 12, 2021·Journal of Experimental Botany·Muhammed Jamsheer KVibha Srivastava

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