PMID: 8603921Mar 1, 1996Paper

The 110-kD spindle pole body component of Saccharomyces cerevisiae is a phosphoprotein that is modified in a cell cycle-dependent manner

The Journal of Cell Biology
D B FriedmanT N Davis

Abstract

Spc110p (Nuf1p) is an essential component of the yeast microtubule organizing center, or spindle pole body (SPB). Asynchronous wild-type cultures contain two electrophoretically distinct isoforms of Spc110p as detected by Western blot analysis, suggesting that Spc110p is modified in vivo. Both isoforms incorporate 32Pi in vivo, suggesting that Spc110p is post-translationally modified by phosphorylation. The slower-migrating 120-kD Spc110p isoform after incubation is converted to the faster-migrating 112-kD isoform after incubation with protein phosphatase PP2A, and specific PP2A inhibitors block this conversion. Thus, additional phosphorylation of Spc110p at serine and/or threonine residues gives rise to the slower-migrating 120-kD isoform. The 120-kD isoform predominates in cells arrested in mitosis by the addition of nocodazole. However, the 120-kD isoform is not detectable in cells grown to stationary phase (G0) or in cells arrested in G1 by the addition of alpha-factor. Temperature-sensitive cell division cycle (cdc) mutations demonstrate that the presence of the 120-kD isoform correlates with mitotic spindle formation but not with SPB duplication. In a synchronous wild-type population, the additional serine/threonine phosp...Continue Reading

References

Sep 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·M L SlaterJ J Gart
Jul 1, 1992·Molecular Biology of the Cell·I FitchB Futcher
Aug 1, 1992·The Journal of Cell Biology·T N Davis
Jun 14, 1991·Cell·J R GeiserT N Davis
Nov 1, 1990·The Journal of Cell Biology·M P Rout, J V Kilmartin
Aug 12, 1988·Cell·R M McCarroll, W L Fangman
Oct 1, 1988·The Journal of Cell Biology·C W JacobsJ R Pringle
Jul 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·S Tabor, C C Richardson
Jan 1, 1974·Cold Spring Harbor Symposia on Quantitative Biology·B Byers, L Goetsch
Sep 1, 1993·Trends in Genetics : TIG·M Winey, B Byers
Jan 18, 1994·Proceedings of the National Academy of Sciences of the United States of America·J M WestendorfL Gerace
Feb 1, 1993·Current Opinion in Cell Biology·M D RoseL L Satterwhite
Apr 1, 1993·Current Opinion in Cell Biology·K Nasmyth

❮ Previous
Next ❯

Citations

Feb 1, 1997·The Journal of Experimental Zoology·S G Sobel
Dec 19, 1998·Cell Motility and the Cytoskeleton·B R Brinkley, T M Goepfert
Jun 29, 2013·Archives of Gerontology and Geriatrics·Koji TomobeYasuyuki Nomura
Oct 16, 1999·Biology of the Cell·M KnopE Schiebel
Mar 10, 2009·The Journal of Biological Chemistry·Eric P HolingerMark Winey
Aug 22, 2001·Molecular Biology of the Cell·F SchaererP Philippsen
Mar 14, 1998·Molecular Biology of the Cell·F MadeoK U Fröhlich
Aug 20, 2003·Molecular Biology of the Cell·Tennessee J YoderTrisha N Davis
Feb 4, 2012·Bioinformatics·Andy C W LaiAlan M Moses
Oct 12, 2004·Annual Review of Cell and Developmental Biology·Sue L Jaspersen, Mark Winey
Jan 1, 1996·Annual Review of Genetics·M A Hoyt, J R Geiser
May 15, 2010·Cell Division·Jorrit M Enserink, Richard D Kolodner
Nov 23, 2000·Proceedings of the National Academy of Sciences of the United States of America·J R NewmanP S Kim
Mar 30, 2001·The Journal of Biological Chemistry·D B FriedmanT N Davis
May 15, 2015·Molecular Biology of the Cell·Yutian PengGeorjana Barnes
Jan 12, 1999·Developmental Biology·G D SmithD P Wolf
May 27, 2016·Molecular Biology of the Cell·Andrew S LyonDavid A Agard
Jun 16, 2018·Biology Open·Kimberly K FongTrisha N Davis
Sep 6, 2002·Oncogene·Mark Winey, Brenda J Huneycutt
Oct 14, 2018·Essays in Biochemistry·Corinne A Tovey, Paul T Conduit

❮ Previous
Next ❯

Related Concepts

Related Feeds

Cell Cycle Pathways

Cell cycle is a complex process regulated by several signal transduction pathways and enzymes. Here is the latest research on regulation of cell cycle and cell cycle pathways.

Cell Checkpoints & Regulators

Cell cycle checkpoints are a series of complex checkpoint mechanisms that detect DNA abnormalities and ensure that DNA replication and repair are complete before cell division. They are primarily regulated by cyclins, cyclin-dependent kinases, and the anaphase-promoting complex/cyclosome. Here is the latest research.