The 14-kDa dynein light chain-family protein Dlc1 is required for regular oscillatory nuclear movement and efficient recombination during meiotic prophase in fission yeast

Molecular Biology of the Cell
F MikiO Niwa

Abstract

A Schizosaccharomyces pombe spindle pole body (SPB) protein interacts in a two-hybrid system with Dlc1, which belongs to the 14-kDa Tctex-1 dynein light chain family. Green fluorescent protein-tagged Dlc1 accumulated at the SPB throughout the life cycle. During meiotic prophase, Dlc1 was present along astral microtubules and microtubule-anchoring sites on the cell cortex, reminiscent of the cytoplasmic dynein heavy chain Dhc1. In a dlc1-null mutant, Dhc1-dependent nuclear movement in meiotic prophase became irregular in its duration and direction. Dhc1 protein was displaced from the cortex anchors and the formation of microtubule bundle(s) that guide nuclear movement was impaired in the mutant. Meiotic recombination in the dlc1 mutant was reduced to levels similar to that in the dhc1 mutant. Dlc1 and Dhc1 also have roles in karyogamy and rDNA relocation during the sexual phase. Strains mutated in both the dlc1 and dhc1 loci displayed more severe defects in recombination, karyogamy, and sporulation than in either single mutant alone, suggesting that Dlc1 is involved in nuclear events that are independent of Dhc1. S. pombe contains a homolog of the 8-kDa dynein light chain, Dlc2. This class of dynein light chain, however, is not ...Continue Reading

References

Feb 1, 1991·Molecular and Cellular Biology·S SilveR Haguenauer-Tsapis
Jan 1, 1991·Methods in Enzymology·S MorenoP Nurse
Sep 1, 1989·Genetics·A S Ponticelli, G R Smith
Apr 8, 1994·Science·Y ChikashigeY Hiraoka
Aug 9, 1996·The Journal of Biological Chemistry·S M KingK K Pfister
Dec 13, 1996·The Journal of Biological Chemistry·S M KingK K Pfister
Sep 30, 1998·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·Y Hiraoka
Feb 27, 1999·Current Opinion in Cell Biology·S Karki, E L Holzbaur
Jan 7, 2000·Experimental Cell Research·K OkazakiO Niwa
Apr 25, 2000·Molecular and Cellular Biology·K TanakaH Okayama
Jan 20, 2001·The Journal of Biological Chemistry·Y K MokM Zhang
Jun 1, 2001·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·A Yamamoto, Y Hiraoka
Jun 27, 2001·The Journal of Cell Biology·A W TaiC H Sung

❮ Previous
Next ❯

Citations

Jul 20, 2007·Chromosoma·Yuji ChikashigeYasushi Hiraoka
Jan 9, 2003·Current Opinion in Cell Biology·N Ronald Morris
Sep 26, 2013·The Journal of Cell Biology·Henning F HornKyle J Roux
Jun 27, 2008·Molecular Biology of the Cell·Christopher A TannerStephen M King
May 2, 2006·Molecular and Cellular Biology·Hsin-Yen Wu, Sean M Burgess
Jul 4, 2008·Annual Review of Cell and Developmental Biology·Needhi Bhalla, Abby F Dernburg
Dec 2, 2006·BMC Genomics·Yongtao Xue-FranzénOlaf Nielsen
Jun 7, 2007·Cell Division·Haruhiko AsakawaYasushi Hiraoka
Jan 28, 2006·PLoS Genetics·K Kevin PfisterElizabeth M C Fisher
Apr 2, 2005·Genetics·Luther Davis, Gerald R Smith
Jan 15, 2014·Cellular and Molecular Life Sciences : CMLS·Ayumu Yamamoto
Apr 25, 2007·Proceedings of the National Academy of Sciences of the United States of America·Johannes SchmittManfred Alsheimer
May 15, 2007·Proceedings of the National Academy of Sciences of the United States of America·Michael N ConradMichael E Dresser
Feb 13, 2013·The Journal of Cell Biology·Masashi YoshidaAyumu Yamamoto
Nov 9, 2011·The Journal of Cell Biology·Jun-Song ChenKathleen L Gould
Jun 14, 2006·The Journal of Cell Biology·Xie TangW Zacheus Cande
Oct 27, 2009·Trends in Cell Biology·Romain Koszul, Nancy Kleckner
Jun 15, 2007·Biology of the Cell·Thibault CourtheouxSylvie Tournier
Apr 14, 2006·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·Hirohisa MasudaYasushi Hiraoka
Oct 31, 2006·Yeast·Jennifer L WellsRamsay J McFarlane
Apr 15, 2015·The Journal of Cell Biology·Kathleen SchefflerPhong T Tran
Mar 19, 2014·Nucleus·Damien Laporte, Isabelle Sagot
May 20, 2015·The Journal of Cell Biology·Masashi YukawaTakashi Toda
Jul 17, 2007·Cell·Kazunori Tomita, Julia Promisel Cooper
Aug 28, 2014·Frontiers in Plant Science·Tongtong Guo, Yuda Fang
Jun 23, 2011·Reproductive Sciences·Xin HuangQing-Yuan Sun
Jul 12, 2002·Yeast
Feb 11, 2005·The Journal of Biological Chemistry·John C WilliamsWayne A Hendrickson

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.