The 19q13 KRAB Zinc-finger protein ZFP82 suppresses the growth and invasion of esophageal carcinoma cells through inhibiting NF-κB transcription and inducing apoptosis

Epigenomics
Lin YeGuosheng Ren

Abstract

To study the epigenetic alternations and biological functions of ZFP82 in esophageal squamous cell carcinoma. Analysis of ZFP82 expression was carried out by quantitative real-time PCR. Cell function was tested by MTS cell proliferation assay, transwell assay and flow cytometry. Gene mechanisms were studied by reverse transcription-PCR (RT-PCR), quantitative real-time PCR, luciferase reporter assay and Western blot. ZFP82 promoter methylation was downregulated in esophageal squamous cell carcinoma. ZFP82 ectopic expression suppressed cell function and regulated NF-κB phosphorylation and genes involved in cell cycle arrest and apoptosis. Moreover, ZFP82 methylation was correlated with age, tumor stage and outcome. ZFP82 is a tumor suppressor and is disrupted by promoter CpG methylation during esophageal carcinogenesis.

References

Apr 28, 2005·Virchows Archiv : an International Journal of Pathology·Xavier DolcetXavier Matias-Guiu
Mar 31, 2007·Nature Methods·Genee Y LeeMina J Bissell
Dec 17, 2009·Expert Opinion on Therapeutic Targets·Yong LinShanling Xu
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Sep 11, 2014·Journal of Cellular Physiology·Anita Roy, Subrata Banerjee
Feb 18, 2015·Oncotarget·Yuko NakagawaKo Sato

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Datasets Mentioned

BETA
KYSE510

Methods Mentioned

BETA
PCR
fluorescence microscopy
transfection
flow cytometry
ChIP

Software Mentioned

CellQuest
Pro Plus
ModFit
Image

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