The 2'-5' RNA ligase of Escherichia coli. Purification, cloning, and genomic disruption.

The Journal of Biological Chemistry
E A Arn, J Abelson

Abstract

An RNA ligase previously detected in extracts of Escherichia coli is capable of joining Saccharomyces cerevisiae tRNA splicing intermediates in the absence of ATP to form a 2'-5' phosphodiester linkage (Greer, C., Javor, B., and Abelson, J. (1983) Cell 33, 899-906). This enzyme specifically ligates tRNA half-molecules containing nucleoside base modifications and shows a preference among different tRNA species. In order to investigate the function of this enzyme in RNA metabolism, the ligase was purified to homogeneity from E. coli lysate utilizing chromatographic techniques and separation of proteins by SDS-polyacrylamide gel electrophoresis. A single polypeptide of approximately 20 kilodaltons exhibited RNA ligase activity. The amino terminus of this protein was sequenced, and the open reading frame (ORF) encoding it was identified by a data base search. This ORF, which encodes a novel protein with a predicted molecular mass of 19.9 kDa, was amplified from E. coli genomic DNA and cloned. ORFs coding for highly similar proteins were detected in Methanococcus jannaschii and Bacillus stearothermophilus. The chromosomal gene encoding RNA ligase in E. coli was disrupted, abolishing ligase activity in cell lysates. Cells lacking lig...Continue Reading

References

May 14, 1992·Nature·B Reinhold-Hurek, D A Shub
Sep 1, 1991·Molecular Microbiology·M VicenteM Aldea
Jan 1, 1990·Methods in Enzymology·C F Evans, D R Engelke
Oct 5, 1990·Journal of Molecular Biology·S F AltschulD J Lipman
Sep 1, 1989·Journal of Bacteriology·C M HamiltonS R Kushner
Oct 1, 1987·Analytical Biochemistry·V M Reyes, J Abelson
Jan 1, 1986·Methods in Enzymology·H SchäggerG von Jagow
Nov 16, 1987·European Journal of Biochemistry·M A TrujilloH V Rickenberg
Nov 15, 1981·Analytical Biochemistry·W WrayR Hancock
Sep 19, 1980·Science·R B WallaceJ Abelson
Jul 28, 1995·Science·R D FleischmannJ M Merrick
Jan 1, 1994·Annual Review of Microbiology·P C Loewen, R Hengge-Aronis
Feb 7, 1995·Biochemistry·K J Hertel, O C Uhlenbeck

❮ Previous
Next ❯

Citations

Mar 20, 2012·Cellular and Molecular Life Sciences : CMLS·Johannes PopowJavier Martinez
Apr 7, 2005·Journal of Molecular Biology·Miyuki Kato-MurayamaShigeyuki Yokoyama
Jun 12, 2003·Journal of Molecular Biology·Miyuki KatoShigeyuki Yokoyama
Oct 23, 1997·Nucleic Acids Research·J KlovinsR C Olsthoorn
Dec 6, 2011·Nucleic Acids Research·Miri DananRotem Sorek
Mar 3, 2006·Acta Crystallographica. Section F, Structural Biology and Crystallization Communications·Dan LiXiao Dong Su
Oct 3, 2006·Acta Crystallographica. Section F, Structural Biology and Crystallization Communications·Cong LiuXiao Dong Su
May 30, 2009·Acta Crystallographica. Section F, Structural Biology and Crystallization Communications·Satoru ShimizuAkio Takénaka
Mar 5, 2011·Microbiology and Molecular Biology Reviews : MMBR·Dea Slade, Miroslav Radman
Mar 26, 2003·Proceedings of the National Academy of Sciences of the United States of America·Yongqing LiuMichael J Daly
Jun 7, 2014·Journal of Interferon & Cytokine Research : the Official Journal of the International Society for Interferon and Cytokine Research·Robert H Silverman, Susan R Weiss
Feb 17, 2005·Journal of Molecular Biology·Yasumitsu SakamotoKazuo T Nakamura
Oct 10, 2015·BMC Structural Biology·Sandrine MoreiraGertraud Burger
Feb 28, 2002·Biochemical and Biophysical Research Communications·Andreas HofmannAlexander Wlodawer
Feb 18, 2012·RNA Biology·Diego Molina-SerranoJosé-Antonio Daròs
Aug 17, 2014·DNA Repair·Leonardo SorciNadia Raffaelli
Sep 15, 2018·Nucleic Acids Research·Yuichiro NomuraSamuel E Butcher
Sep 17, 1998·The Journal of Biological Chemistry·P GenschikW Filipowicz
Feb 1, 2017·PloS One·Matti Myllykoski, Petri Kursula
Nov 27, 1999·The Journal of Biological Chemistry·E BillyW Filipowicz
Aug 20, 2019·Biochimie·Shuhei MitsutomiChikara Kaito
Aug 28, 1997·Biochemical and Biophysical Research Communications·I Gomes, R Gupta
Sep 8, 1998·Microbiology and Molecular Biology Reviews : MMBR·M K Berlyn
Sep 23, 2014·RNA·Barbara S RemusStewart Shuman

❮ Previous
Next ❯

Related Concepts

Related Feeds

ASBMB Publications

The American Society for Biochemistry and Molecular Biology (ASBMB) includes the Journal of Biological Chemistry, Molecular & Cellular Proteomics, and the Journal of Lipid Research. Discover the latest research from ASBMB here.