The α2 isoform of the Na,K-pump is important for intercellular communication, agonist-induced contraction, and EDHF-like response in rat mesenteric arteries

American Journal of Physiology. Heart and Circulatory Physiology
Vladimir V MatchkovChristian Aalkjaer

Abstract

The specific role of different isoforms of the Na,K-pump in the vascular wall is still under debate. We have previously suggested that the α(2) isoform of the Na,K-pump (α(2)), Na(+), Ca(2+)-exchange (NCX), and connexin43 form a regulatory microdomain in smooth muscle cells (SMCs), which controls intercellular communication and contractile properties of the vascular wall. We have tested this hypothesis by downregulating α(2) in cultured SMCs and in small arteries with siRNA in vivo. Intercellular communication was assessed by using membrane capacitance measurements. Arteries transfected in vivo were tested for isometric and isobaric force development in vitro; [Ca(2+)](i) was measured simultaneously. Cultured rat SMCs were well-coupled electrically, but 10 μM ouabain uncoupled them. Downregulation of α(2) reduced electrical coupling between SMCs and made them insensitive to ouabain. Downregulation of α(2) in small arteries was accompanied with significant reduction in NCX expression. Acetylcholine-induced relaxation was not different between the groups, but the endothelium-dependent hyperpolarizing factor-like component of the response was significantly diminished in α(2)-downregulated arteries. Micromolar ouabain reduced in a ...Continue Reading

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Citations

Oct 29, 2013·Pflügers Archiv : European journal of physiology·Vibeke Secher DamVladimir Matchkov
Jan 13, 2016·The Journal of General Physiology·Violetta V KravtsovaIgor I Krivoi
Jun 3, 2016·Frontiers in Physiology·Vladimir V Matchkov, Igor I Krivoi
Jun 2, 2017·The Journal of Physiology·Jakob NyvadVladimir V Matchkov
Jan 27, 2017·American Journal of Physiology. Cell Physiology·Lise HangaardVladimir V Matchkov
Jun 26, 2018·Acta Physiologica·R Schubert
Feb 16, 2019·Experimental Physiology·Christian StaehrVladimir V Matchkov
Feb 25, 2014·American Journal of Physiology. Heart and Circulatory Physiology·Olga KudryavtsevaChristian Aalkjær
Sep 10, 2013·American Journal of Physiology. Heart and Circulatory Physiology·Zhuoming LiPaul M Vanhoutte
Mar 8, 2018·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·Christian StaehrVladimir V Matchkov
Mar 17, 2015·The Journal of Physiology·Michael J ShattockZi-Jian Xie

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