The 2'-OH group at the group II intron terminus acts as a proton shuttle.

Nature Chemical Biology
Michael RoitzschA M Pyle

Abstract

Group II introns are self-splicing ribozymes that excise themselves from precursor RNAs and catalyze the joining of flanking exons. Excised introns can behave as parasitic RNA molecules: they can catalyze their own insertion into DNA and RNA via a reverse splicing reaction. Previous studies have identified mechanistic roles for various functional groups located in the catalytic core of the intron and within target molecules. Here we introduce a new method for synthesizing long RNA molecules with a modified nucleotide at the 3' terminus. This modification allows us to examine the mechanistic role of functional groups adjacent to the reaction nucleophile. During reverse splicing, the 3'-OH group of the intron terminus attacks the phosphodiester linkage of spliced exon sequences. Here we show that the adjacent 2'-OH group on the intron terminus plays an essential role in activating the nucleophile by stripping away a proton from the 3'-OH and then shuttling it from the active site.

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Citations

Apr 10, 2012·Nature Structural & Molecular Biology·Russell T ChanNavtej Toor
Oct 14, 2016·Journal of the American Chemical Society·Benjamin W NoffkeLiang-Shi Li
May 20, 2011·Chemistry : a European Journal·Tuomas Lönnberg
May 3, 2018·Beilstein Journal of Organic Chemistry·Satu MikkolaHarri Lönnberg
Feb 12, 2020·Wiley Interdisciplinary Reviews. RNA·Daniel B Haack, Navtej Toor
Jul 29, 2020·The Journal of Biological Chemistry·Pratik Rajendra PatilBalaji Prakash
Jun 7, 2020·Nature Communications·Jacopo ManigrassoMarco Marcia

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