The 2.0 A structure of a cross-linked complex between snowdrop lectin and a branched mannopentaose: evidence for two unique binding modes

Structure
C S Wright, G Hester

Abstract

Galanthus nivalis agglutinin (GNA), a mannose-specific lectin from snowdrop bulbs, is a tetrameric member of the family of Amaryllidaceae lectins that exhibit antiviral activity towards HIV. Its subunits are composed of three pseudo-symmetrically related beta sheet domains, each with a conserved mannose-binding site. Crystal structures of monosaccharide and disaccharide complexes of GNA have revealed that all 12 binding sites of the tetramer are functional, and that the degree of occupancy is dependent on the availability of subsidiary interactions from neighboring subunits. The complex of GNA with a branched mannopentaose ((Manalpha1,6-(alpha1, 3-Man)Man-alpha1,6-(alpha1,3-Man)Man) described here simulates a more biologically relevant complex. Two unique mannopentaose binding modes co-exist in the tetragonal structure (1 subunit/asymmetric unit) of the complex. In one, the conserved monosaccharide-binding pocket in domain 1 (CRD 1) is utilized for cross-linkage of twofold related GNA dimers by the outer 3,6 tri-Man arm, which alternates between two orientations consistent with crystal symmetry. Inter-linked dimers assemble helically along the 41 crystal axis forming a pore-like structure. In the second binding mode, the comple...Continue Reading

References

May 25, 1977·Journal of Molecular Biology·F C BernsteinM Tasumi
Nov 15, 1991·European Journal of Biochemistry·E J Van DammeW J Peumans
Mar 1, 1991·Acta Crystallographica. Section A, Foundations of Crystallography·T A JonesM Kjeldgaard
Nov 1, 1990·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·N Sharon, H Lis
Jan 1, 1990·European Biophysics Journal : EBJ·E W WootenT W Rademacher
Oct 13, 1989·Science·N Sharon, H Lis
Jan 1, 1974·International Review of Cytology·G L Nicolson
Jan 1, 1995·Annual Review of Biophysics and Biomolecular Structure·J M Rini
Aug 1, 1994·Nature Structural Biology·R J WoodsR A Dwek
Jun 1, 1995·Nature Structural Biology·K Drickamer
Dec 1, 1994·Nature Structural Biology·Y BourneC Cambillau
Aug 1, 1994·Glycobiology·J Favero
Jan 1, 1993·Annual Review of Cell Biology·K Drickamer, M E Taylor
Jun 1, 1993·Trends in Biochemical Sciences·N Sharon
Jan 12, 1996·The Journal of Biological Chemistry·K K NgW I Weis
Jan 12, 1996·The Journal of Biological Chemistry·J H Naismith, R A Field
Sep 1, 1994·Acta Crystallographica. Section D, Biological Crystallography·UNKNOWN Collaborative Computational Project, Number 4
Jan 23, 1987·Science·A T BrüngerM Karplus

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Citations

Nov 5, 1997·Current Opinion in Structural Biology·A Imberty
Nov 5, 1997·Current Opinion in Structural Biology·C S Wright
Dec 23, 1999·Current Opinion in Structural Biology·M Vijayan, N Chandra
Jun 20, 2002·Journal of Biotechnology·Claus W von der LiethThisbe K Lindhorst
Nov 18, 2009·Journal of the American Chemical Society·Wei HuangLai-Xi Wang
Jan 23, 1999·European Journal of Biochemistry·E J Van DammeW J Peumans
Aug 10, 2000·European Journal of Biochemistry·E J Van DammeW J Peumans
Jun 14, 2012·The Journal of Biological Chemistry·Raquel Guimarães BenevidesAnne Imberty
Jun 9, 2007·Plant Physiology·Abel Garcia-PinoRemy Loris
Jul 31, 2013·Acta Crystallographica. Section D, Biological Crystallography·Alok SharmaM Vijayan
Apr 21, 2011·Acta Crystallographica. Section F, Structural Biology and Crystallization Communications·D Wade Abbott, Alisdair Boraston
May 25, 1999·Protein Science : a Publication of the Protein Society·E García-Hernández, A Hernández-Arana
Jul 20, 1999·Annual Review of Biophysics and Biomolecular Structure·C A BushA Imberty
Mar 8, 2013·PLoS Pathogens·Maarten G K GhequireRené De Mot
Oct 13, 2015·Journal of Biomolecular Structure & Dynamics·Priyadarshini P Pai, Sukanta Mondal
Mar 1, 2015·Expert Opinion on Therapeutic Targets·Priyamvada AcharyaPeter D Kwong
Oct 6, 1999·Current Opinion in Structural Biology·J BouckaertR Loris
Dec 2, 2004·Progress in Biophysics and Molecular Biology·Istvan Botos, Alexander Wlodawer
Mar 30, 2006·Antiviral Research·J Balzarini
Dec 19, 2000·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·K SamesU Schumacher
Jul 27, 2011·Glycobiology·Kartika N ShettyK Suguna
Jan 13, 2019·International Journal of Molecular Sciences·Annick BarrePierre Rougé
Aug 26, 2016·Glycobiology·Patricia R PereiraJeanne A Stuckey
Aug 16, 2001·The Journal of Biological Chemistry·M GoelD M Salunke
Feb 19, 2002·Chemical Reviews·Halina Lis, Nathan Sharon

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