The 21-residue surfactant peptide (LysLeu4)4Lys(KL4) is a transmembrane alpha-helix with a mixed nonpolar/polar surface

FEBS Letters
M GustafssonJ Johansson

Abstract

The 21-residue peptide KLLLLKLLLLKLLLLKLLLLK (KL4) has been synthesized and analyzed regarding its secondary structure and orientation in lipid environments. Fourier transform infrared and circular dichroism spectroscopy shows that the peptide exhibits approximately 80% alpha-helical content both in dodecylphosphocholine micelles and in 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC)/phosphatidylglycerol (PG) 7:3 (w/w) bilayers. The positively charged lysine residues are evenly distributed over the entire, otherwise nonpolar, circumference of the helix. This is in sharp contrast to the uneven distribution of polar and nonpolar residues in amphipathic helices. Fourier transform infrared spectroscopy of the peptide inserted in DPPC/PG bilayers shows that the helical axis is oriented parallel to the lipid acyl chains. These data do not support a previous hypothesis that the KL4 peptide interacts with peripheral parts of a phospholipid monolayer and mimics the pulmonary surfactant protein SP-B, which is composed of several amphipathic alpha-helices. KL4 accelerates the spreading of phospholipid mixtures at an air/water interface but does so less efficiently than other transmembranous helical polypeptides studied.

References

Jan 15, 1992·European Journal of Biochemistry·G VandenbusscheJ M Ruysschaert
Apr 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·T CurstedtH Jörnvall
Dec 12, 1980·Biochimica Et Biophysica Acta·C BöschK Wüthrich
Jun 1, 1994·Nature Structural Biology·S C Li, C M Deber
Feb 1, 1994·The European Respiratory Journal·J JohanssonB Robertson
Jan 1, 1996·American Journal of Respiratory and Critical Care Medicine·C G CochraneM S Alberts

❮ Previous
Next ❯

Citations

Jan 15, 2004·American Journal of Respiratory Medicine : Drugs, Devices, and Other Interventions·Sean B Ainsworth, David W A Milligan
Feb 25, 2014·Advances in Colloid and Interface Science·Mustapha LhorChristian Salesse
Feb 26, 2000·Molecular Medicine Today·B RobertsonT Curstedt
Mar 15, 1997·European Journal of Biochemistry·J Johansson, T Curstedt
Feb 22, 2001·Annual Review of Physiology·T E Weaver, J J Conkright
Jun 30, 2005·Biology of the Neonate·Tore Curstedt, Jan Johansson
Oct 6, 1997·American Journal of Respiratory and Critical Care Medicine·F J WaltherA J Waring
Mar 14, 2016·Current Opinion in Chemical Biology·Otonye Braide-MoncoeurJoanna R Long
May 24, 2017·Nature Communications·Nina KronqvistJan Johansson
Mar 28, 2017·Biochimica Et Biophysica Acta. Biomembranes·Mercedes EchaideJesus Perez-Gil
Dec 1, 2005·The Journal of Peptide Research : Official Journal of the American Peptide Society·A J WaringJ A Zasadzinski
Dec 21, 2007·The Journal of Biological Chemistry·Mohammed SaleemHans-Joachim Galla
Mar 12, 2013·Expert Review of Clinical Pharmacology·Brian K Jordan, Steven M Donn
Oct 1, 2007·The Journal of Pediatric Pharmacology and Therapeutics : JPPT : the Official Journal of PPAG·Sunil K Sinha
Sep 26, 2000·Molecular Genetics and Metabolism·F J WaltherA J Waring
Nov 14, 1998·Biochimica Et Biophysica Acta·J Johansson
Nov 14, 1998·Biochimica Et Biophysica Acta·T E Weaver
Jan 25, 2005·Chemistry & Biology·Shannon L SeurynckAnnelise E Barron
Feb 9, 2007·Colloids and Surfaces. B, Biointerfaces·Shannon L Seurynck-ServossAnnelise E Barron
Apr 3, 2007·Journal of the American Chemical Society·Lisa A CapriottiJoel P Schneider
Nov 3, 2009·Biochimica Et Biophysica Acta·Mohammed Saleem, Hans-Joachim Galla

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.