The α2AR/Caveolin-1/p38MAPK/NF-κB axis explains dexmedetomidine protection against lung injury following intestinal ischaemia-reperfusion.

Journal of Cellular and Molecular Medicine
Lin XuXuekang Zhang

Abstract

Intestinal ischaemia-reperfusion (I/R) injury can result in acute lung injury due to ischaemia and hypoxia. Dexmedetomidine (Dex), a highly selective alpha2-noradrenergic receptor (α2AR) agonist used in anaesthesia, is reported to regulate inflammation in organs. This study aimed to investigate the role and mechanism of Dex in lung injury caused by intestinal I/R. After establishing a rat model of intestinal I/R, we measured the wet-to-dry specific gravity of rat lungs upon treatments with Dex, SB239063 and the α2AR antagonist Atipamezole. Moreover, injury scoring and histopathological studies of lung tissues were performed, followed by ELISA detection on tumour necrosis factor-α (TNF-α), interleukin (IL)-1β and IL-6 expression. Correlation of Caveolin-1 (Cav-1) protein expression with p38, p-p38, p-p65 and p65 in rat lung tissues was analysed, and the degree of cell apoptosis in lung tissues after intestinal I/R injury was detected by TUNEL assay. The lung injury induced by intestinal I/R was a dynamic process. Moreover, Dex had protective effects against lung injury by mediating the expression of Cal-1 and α2A -AR. Specifically, Dex promoted Cav-1 expression via α2A -AR activation and mitigated intestinal I/R-induced lung inj...Continue Reading

References

Dec 17, 2005·American Journal of Respiratory Cell and Molecular Biology·Xiao Mei WangAugustine M K Choi
Mar 12, 2011·The Journal of Surgical Research·Dao-Feng BenZhao-Fan Xia
Jun 7, 2011·Postgraduate Medical Journal·A DushianthanR Cusack
Jan 31, 2013·Critical Care : the Official Journal of the Critical Care Forum·Shingo MatsuoPing Wang
Jun 21, 2013·Archives of Pathology & Laboratory Medicine·Timothy Craig Allen, Anna Kurdowska
Nov 27, 2015·Pharmacological Research : the Official Journal of the Italian Pharmacological Society·Jaime A RiquelmeSergio Lavandero
Mar 15, 2016·World Journal of Gastroenterology : WJG·Joep GrootjansJoep P M Derikx
Jun 20, 2017·Journal of Clinical Anesthesia·Lianxiang JiangZeping Dai
Jun 15, 2019·Journal of Cellular and Molecular Medicine·Guo-Wei TuLi-Ming Lu
Jan 22, 2020·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Shihong WenWenqi Huang
Apr 27, 2020·Experimental and Molecular Pathology·Xiao-Ming LiuXue-Kang Zhang
Oct 13, 2020·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Na HeShouwei Yue

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