PMID: 11904223Mar 21, 2002Paper

The 3-D structure of microsomal glutathione transferase 1 at 6 A resolution as determined by electron crystallography of p22(1)2(1) crystals

Biochimica Et Biophysica Acta
Peter J HolmHans Hebert

Abstract

Pure solubilised microsomal glutathione transferase 1 (MGST1) forms well-ordered two-dimensional (2-D) crystals of two different symmetries, one orthorhombic (p22(1)2(1)) and one hexagonal (p6), both diffracting electrons to a resolution beyond 3 A. A three-dimensional (3-D) map has previously been calculated to 6 A resolution from the hexagonal crystal form. From orthorhombic crystals we have now calculated a 6 A 3-D reconstruction displaying three repeats of four rod-like densities. These are inclined relative to the normal of the membrane plane and consistent with arising from a left-handed four-helix bundle fold. The rendered volume clearly displays the same structural features as the map previously calculated from the p6 crystal type including similar lengths and substructure of the helices, but several distinguishing features do exist. The helices are more tilted in the map calculated from the orthorhombic crystals indicating conformational flexibility. Density present on the cytosolic side is consistent with the location of the active site. In addition, the current map displays the noted similarity to subunit I of cytochrome c oxidase.

References

Mar 1, 1991·Acta Crystallographica. Section A, Foundations of Crystallography·T A JonesM Kjeldgaard
Jan 1, 1982·Ultramicroscopy·R Hegerl, A Altbauer
Feb 16, 1994·Biochimica Et Biophysica Acta·C AnderssonR Morgenstern
Mar 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·D W NicholsonA W Ford-Hutchinson
Jan 1, 1996·Journal of Structural Biology·R A CrowtherJ M Smith
Aug 15, 1997·The Biochemical Journal·T H Sun, R Morgenstern
Sep 1, 1994·Acta Crystallographica. Section D, Biological Crystallography·UNKNOWN Collaborative Computational Project, Number 4

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Citations

Sep 3, 2013·European Biophysics Journal : EBJ·Timothy A CrossAnthony Watts
May 10, 2011·Annual Review of Biochemistry·Nelson P Barrera, Carol V Robinson
Jun 30, 2006·Journal of Molecular Biology·Peter J HolmHans Hebert
Jul 30, 2011·The Biochemical Journal·Shan HeLuhua Lai
Nov 9, 2004·Structure·Ingeborg Schmidt-KreyWerner Kühlbrandt
Apr 12, 2005·Annual Review of Pharmacology and Toxicology·John D HayesIan R Jowsey
Jan 23, 2016·Journal of Medicinal Chemistry·Andreas KoeberleOliver Werz
Nov 3, 2005·Current Opinion in Structural Biology·Aaron J Oakley
Mar 23, 2006·Journal of Cellular Biochemistry·Jesper SvartzSven Hammarström
Mar 30, 2005·The FEBS Journal·Anders BresellRalf Morgenstern
Dec 4, 2016·Biochimica Et Biophysica Acta. Biomembranes·Miyuki ShimojiEinar Hallberg
Sep 20, 2007·Pharmacological Reviews·Bengt SamuelssonPer-Johan Jakobsson
Jan 17, 2020·Critical Reviews in Toxicology·Patrick E Hanna, M W Anders
Nov 30, 2005·The Journal of Biological Chemistry·Yanbin JiBrian M Bennett
Jul 28, 2012·The Journal of Experimental Biology·Rafael Ortiz-RodríguezClaudia Wiegand
Dec 8, 2004·Archives of Biochemistry and Biophysics·Laura S Busenlehner, Richard N Armstrong
Mar 1, 2008·Biochemical and Biophysical Research Communications·Hiromi DaiyasuHiroyuki Toh
May 7, 2009·Archives of Biochemistry and Biophysics·Johan AlanderRalf Morgenstern

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