The 3' terminal colicin fragment of Escherichia coli 16S ribosomal RNA. Conformational details revealed by enzymic and chemical probing.

Journal of Biomolecular Structure & Dynamics
H A Heus, P H Van Knippenberg

Abstract

The conformation of the colicin fragment of E. coli 16S rRNA was probed with various nucleases and with the adenosine-specific reagent diethylpyrocarbonate (DEP). The results confirm the presence of a stable central hairpin in the colicin fragment and a weaker additional secondary structure involving the regions 5' and 3' to this hairpin. By monitoring DEP accessibility at various stages of heat-denaturation sequential unfolding of individual base pairs was followed. In agreement with previous results it could be shown that dimethylation of the two adjacent adenosines in the hairpin loop (a feature in virtually all ribosomes) leads to a destabilization of the hairpin helix. Accessibilities of G residues, involved in the weaker additional secondary structure is anomalous. One G residue is sensitive to the single strand specific RNase T1 and insensitive to DEP, while the situation is reversed for the adjoining G residue. The strong reaction of the latter G-residue with DEP is unusual and indicates a very special conformation.

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Jul 10, 1998·Structure·J P Rife, P B Moore
Jan 1, 1994·Tijdschrift voor economische en sociale geografie = Journal of economic and social geography = Revue de géographie économique et humaine = Zeitschrift für ökonomische und soziale Geographie = Revista de geografía económica y social·C Wessels, J M Beck

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