The 3'-untranslated region of chloroplast psbA mRNA stabilizes binding of regulatory proteins to the leader of the message

The Journal of Biological Chemistry
Yael Katz, A Danon

Abstract

The 5'-leader and 3'-tail of chloroplast mRNAs have been suggested to play a role in posttranscriptional regulation of expression of the message. The regulation is thought to be mediated, at least in part, by regulatory proteins that are encoded by the nuclear genome and targeted to the chloroplast where they interact with chloroplast mRNAs. Previous studies identified high affinity binding of the 5'-untranslated region (UTR) of the chloroplast psbA mRNA by Chlamydomonas reinhardtii proteins. Here we tested whether the 3'-UTR of psbA mRNA alone or linked in cis with the 5'-UTR of the mRNA affects the high affinity binding of the message in vitro. We did not detect high affinity binding that is unique to the 3'-UTR. However, we show that the cis-linked 3'-UTR increases the stability of the 5'-UTR binding complex. This effect could provide a means for translational discrimination against mRNAs that are incorrectly processed.

References

Oct 1, 1992·Current Opinion in Genetics & Development·L Lindahl, A Hinnebusch
Dec 1, 1965·Proceedings of the National Academy of Sciences of the United States of America·D S Gorman, R P Levine
Oct 1, 1994·The Plant Journal : for Cell and Molecular Biology·J M Staub, P Maliga
Sep 1, 1996·Molecular Microbiology·T Franch, K Gerdes
Nov 12, 1996·Proceedings of the National Academy of Sciences of the United States of America·I LisitskyG Schuster
Apr 16, 1998·Proceedings of the National Academy of Sciences of the United States of America·C B YohnS P Mayfield
Jul 22, 1998·Molecular and Cellular Biology·R RottG Schuster
Oct 3, 1999·The Plant Journal : for Cell and Molecular Biology·R G DragerD B Stern
Apr 12, 2000·The Plant Journal : for Cell and Molecular Biology·F E VaistijJ D Rochaix
Jun 29, 2000·Annual Review of Biochemistry·A C GingrasN Sonenberg
Aug 18, 2000·Biochimie·R A MondeD B Stern
Oct 12, 2000·Molecular and Cellular Biology·F Ossenbühl, J Nickelsen

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Citations

Jul 31, 2007·Photosynthesis Research·Julia Marín-NavarroStephen P Mayfield
May 14, 2005·Antioxidants & Redox Signaling·Thomas Pfannschmidt, Karsten Liere

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